CAJ | 학술논문

目的：观察Cx43蛋白在丰富环境改善脑外伤(TBI)大鼠学习记忆能力中的作用。方法选用成年Sprague-Dawley大鼠，采用液压颅脑损伤仪制作TBI模型，将模型大鼠分为丰富环境刺激组(A组)、常规饲养组(B组)、Cx43特异性反义寡聚核苷酸(Cx43-ASODN)+丰富环境刺激组(C组)和乱序ODN(scrambled sequence ODN)+丰富环境刺激组(D组)，每组6只。另设6只正常大鼠作为对照组。C组和D组分别在海马区注射Cx43-ASODN(2μl/d/rat,1.5 mmol/L)和ScrbASODN(2μl/d/rat,1.5 mmol/L)。饲养14 d，采用水迷宫测试评估大鼠的学习记忆能力。结果 B组大鼠潜伏期大于对照组(P<0.05)，穿越次数少于对照组(P<0.05)。A组潜伏期小于B组(P<0.05)，且从第9天起与对照组比较无显著性差异(P>0.05)；穿越平台次数多于B组，与对照组比较无显著性差异(P>0.05)。C组大鼠的潜伏期大于A组(P<0.05)，穿越平台次数少于A组(P<0.05)。结论 Cx43蛋白参与丰富环境改善TBI大鼠学习记忆能力的调控。
목적：관찰Cx43단백재봉부배경개선뇌외상(TBI)대서학습기억능력중적작용。방법선용성년Sprague-Dawley대서，채용액압로뇌손상의제작TBI모형，장모형대서분위봉부배경자격조(A조)、상규사양조(B조)、Cx43특이성반의과취핵감산(Cx43-ASODN)+봉부배경자격조(C조)화란서ODN(scrambled sequence ODN)+봉부배경자격조(D조)，매조6지。령설6지정상대서작위대조조。C조화D조분별재해마구주사Cx43-ASODN(2μl/d/rat,1.5 mmol/L)화ScrbASODN(2μl/d/rat,1.5 mmol/L)。사양14 d，채용수미궁측시평고대서적학습기억능력。결과 B조대서잠복기대우대조조(P<0.05)，천월차수소우대조조(P<0.05)。A조잠복기소우B조(P<0.05)，차종제9천기여대조조비교무현저성차이(P>0.05)；천월평태차수다우B조，여대조조비교무현저성차이(P>0.05)。C조대서적잠복기대우A조(P<0.05)，천월평태차수소우A조(P<0.05)。결론 Cx43단백삼여봉부배경개선TBI대서학습기억능력적조공。
Objective To explore the effect of Cx43 protein on improvement of learning and memory ability induced by enriched envi-ronment (EE) in rats with traumatic brain injury (TBI). Methods TBI model was made by fluid percussion injury (FPI) in Sprague-Dawley rats. The TBI rats were divided into EE group (A), standard housing (ST) group (B), Cx43 specific antisense oligonucleotides (Cx43 ASODN)+EE group (C) and scrambled sequence ODN+EE group (D) with 6 rats in each group. Another 6 normal rats were taken as the control group. Groups C and D were given hippocampal microinjection of Cx43-ASODN (2μl/d/rat, 1.5 mmol/L) and ScrbASODN (2μl/d/rat, 1.5 mmol/L) respectively. Morris water maze was used to evaluated the learning and memory ability. Results The latency was longer and the traversing times was less in group B than in the control group (P<0.05). The latency was shorter in group A than in group B (P<0.05), and there was no significant difference between group A and the control group (P>0.05) from the 9th day after injury. The traversing times was more in group A than in group B and there was no significant difference between group A and the control group (P>0.05). The la-tency was longer and the traversing times was less in group C than in group A (P<0.05). Conclusion Cx43 protein may participate in the im-provement of the learning and memory ability induced by EE in rats with TBI.