中国康复理论与实践
中國康複理論與實踐
중국강복이론여실천
CHINESE JOURNAL OF REHABILITATION THEORY & PRACTICE
2014年
10期
919-923
,共5页
杨俊锋%张亚峰%马勇%吴毛%郭杨%顾晓林%王建伟
楊俊鋒%張亞峰%馬勇%吳毛%郭楊%顧曉林%王建偉
양준봉%장아봉%마용%오모%곽양%고효림%왕건위
脊髓损伤%勿动蛋白受体%轴突再生%大鼠
脊髓損傷%勿動蛋白受體%軸突再生%大鼠
척수손상%물동단백수체%축돌재생%대서
spinal cord injury%Nogo receptor%axonal regeneration%rats
目的:观察大鼠脊髓损伤后勿动蛋白受体(NgR)在脊髓组织中的动态表达变化。方法108只Sprague-Dawley大鼠随机分成正常组、假手术组和模型组,每组36只,其中模型组采用改良Allen法造模。3组分别于干预后24 h、3 d、7 d、14 d处死大鼠,每组9只,以免疫组化及Western blotting检测各组大鼠脊髓组织中NgR表达的变化,以荧光定量PCR检测NgR mRNA表达变化。结果正常组、假手术组各时间点NgR表达无明显变化(P>0.05)。模型组在损伤后24 h NgR及其mRNA表达较低,3 d后下降至最低,7 d后迅速上升达到高峰,14 d时有所下降。与正常组比较,模型组在损伤后各时间点,NgR免疫组化及Western blotting蛋白表达差异均有统计学意义(P<0.05);与假手术组比较,模型组在损伤后各时间点,NgR mRNA表达差异均有统计学意义(P<0.01)。结论大鼠脊髓损伤后,NgR及其mRNA表达均在7 d时上升至峰值,并较长时间保持高水平表达。这可能是造成脊髓损伤后轴突再生困难的重要原因之一。
目的:觀察大鼠脊髓損傷後勿動蛋白受體(NgR)在脊髓組織中的動態錶達變化。方法108隻Sprague-Dawley大鼠隨機分成正常組、假手術組和模型組,每組36隻,其中模型組採用改良Allen法造模。3組分彆于榦預後24 h、3 d、7 d、14 d處死大鼠,每組9隻,以免疫組化及Western blotting檢測各組大鼠脊髓組織中NgR錶達的變化,以熒光定量PCR檢測NgR mRNA錶達變化。結果正常組、假手術組各時間點NgR錶達無明顯變化(P>0.05)。模型組在損傷後24 h NgR及其mRNA錶達較低,3 d後下降至最低,7 d後迅速上升達到高峰,14 d時有所下降。與正常組比較,模型組在損傷後各時間點,NgR免疫組化及Western blotting蛋白錶達差異均有統計學意義(P<0.05);與假手術組比較,模型組在損傷後各時間點,NgR mRNA錶達差異均有統計學意義(P<0.01)。結論大鼠脊髓損傷後,NgR及其mRNA錶達均在7 d時上升至峰值,併較長時間保持高水平錶達。這可能是造成脊髓損傷後軸突再生睏難的重要原因之一。
목적:관찰대서척수손상후물동단백수체(NgR)재척수조직중적동태표체변화。방법108지Sprague-Dawley대서수궤분성정상조、가수술조화모형조,매조36지,기중모형조채용개량Allen법조모。3조분별우간예후24 h、3 d、7 d、14 d처사대서,매조9지,이면역조화급Western blotting검측각조대서척수조직중NgR표체적변화,이형광정량PCR검측NgR mRNA표체변화。결과정상조、가수술조각시간점NgR표체무명현변화(P>0.05)。모형조재손상후24 h NgR급기mRNA표체교저,3 d후하강지최저,7 d후신속상승체도고봉,14 d시유소하강。여정상조비교,모형조재손상후각시간점,NgR면역조화급Western blotting단백표체차이균유통계학의의(P<0.05);여가수술조비교,모형조재손상후각시간점,NgR mRNA표체차이균유통계학의의(P<0.01)。결론대서척수손상후,NgR급기mRNA표체균재7 d시상승지봉치,병교장시간보지고수평표체。저가능시조성척수손상후축돌재생곤난적중요원인지일。
Objective To observe the dynamic expression of Nogo receptor (NgR) in spinal cord of rats after spinal cord injury. Meth-ods 108 Sprague-Dawley rats were randomly assigned into normal group, sham operated group and model group, with 36 rats in each group. The model of spinal cord injury was established with the modified Allen's method. The rats were killed 24 h, 3 days, 7 days and 14 days re-spectively after intervention (9 rats from each group), and expression of NgR in the spinal cord tissue of the rats was detected with immuno-histochemistry and Western blotting, and expression of NgR mRNA was detected with fluorescence quantitative PCR. Results There was no significant change in the expression of NgR in the normal group and the sham operated group (P>0.05). The expression of protein and mRNA of NgR was less in the model group 24 h after modeling, dropped to the lowest on the 3rd day, then rapidly peaked on the 7th day, and gradually declined on the 14th day after spinal cord injury. Compared with the normal group, there were significant differences in ex-pression of NgR in immunohistochemistry and Western blotting in the model group at each time point after spinal cord injury (P<0.05). Compared with the sham operated group, there were significant differences in expression of NgR mRNA in the model group at each time point after spinal cord injury (P<0.01). Conclusion The expression of NgR and mRNA peaks on the 7th day after spinal cord injury in the rats, and maintains at high level for a long time, which may associated with the difficulty of axonal regeneration after spinal cord injury.