CAJ | 학술논문

目的：探讨脑肠肽Ghrelin对内毒素（LPS）诱导急性肺损伤（ALI）大鼠肺血管通透性的影响。方法 SD大鼠随机分为生理盐水对照组、ALI模型组及Ghrelin治疗组，每组12只，静脉注射LPS（5 mg／kg）制备ALI模型，Ghrelin治疗组在LPS注射30 min后经腹腔给予Ghrelin （40 nmol／kg），对照组给予等体积生理盐水。观察6 h后处死动物，取肺组织计算肺湿／干（W／D）及行病理学检查，肺组织匀浆检测伊文思蓝（ EB）提取含量和肺组织髓过氧化物（ MPO）活性，收集肺泡灌洗液（ BALF）检测其中炎性细胞总数、百分比及蛋白含量，取动脉血行血气分析。结果与ALI模型组比较，Ghrelin治疗组肺W／D、EB提取含量及MPO活性均明显降低（ P＜0.05）， BALF中炎性细胞总数及蛋白含量亦明显降低（ P＜0．05）。血气结果比较，Ghrelin治疗组较ALI模型组PaO2明显上升（P＜0．01），PaCO2明显下降（P＜0．05），pH值明显升高（P＜0.05）。病理检测提示，Ghrelin治疗组肺组织损伤程度较ALI模型组明显减轻。结论 Ghrelin可降低ALI肺血管通透性，对LPS诱导的ALI大鼠发挥保护作用。
목적：탐토뇌장태Ghrelin대내독소（LPS）유도급성폐손상（ALI）대서폐혈관통투성적영향。방법 SD대서수궤분위생리염수대조조、ALI모형조급Ghrelin치료조，매조12지，정맥주사LPS（5 mg／kg）제비ALI모형，Ghrelin치료조재LPS주사30 min후경복강급여Ghrelin （40 nmol／kg），대조조급여등체적생리염수。관찰6 h후처사동물，취폐조직계산폐습／간（W／D）급행병이학검사，폐조직균장검측이문사람（ EB）제취함량화폐조직수과양화물（ MPO）활성，수집폐포관세액（ BALF）검측기중염성세포총수、백분비급단백함량，취동맥혈행혈기분석。결과여ALI모형조비교，Ghrelin치료조폐W／D、EB제취함량급MPO활성균명현강저（ P＜0.05）， BALF중염성세포총수급단백함량역명현강저（ P＜0．05）。혈기결과비교，Ghrelin치료조교ALI모형조PaO2명현상승（P＜0．01），PaCO2명현하강（P＜0．05），pH치명현승고（P＜0.05）。병리검측제시，Ghrelin치료조폐조직손상정도교ALI모형조명현감경。결론 Ghrelin가강저ALI폐혈관통투성，대LPS유도적ALI대서발휘보호작용。
Objective To observe the effects of ghrelin on pulmonary capillary permeability of acute lung injury ( ALI) caused by LPS.Methods Male SD rats were randomly divided into three groups:saline control group which received normal saline , ALI group which received LPS (5 mg/kg) to induce ALI model, Ghrelin therapy group were intraperitoneally injected with ghrelin (40 nmol/kg) 30 min after LPS challenge.Rats were sacrificed at 6 h after LPS injection.Arterial blood was collected for determination blood gas . Lung tissue was removed and the right lung was lavaged to collected bronchoalveolar lavage fluid ( BALF) for determination of protein content , and differential inflammatory cell counts . The left lung was used for microscopic examination , wet/dry lung weight ratio , myeloperoxidase (MPO) activity and Evans Blue (EB) content.Results In ghrelin therapy group, lung weight ratio , EB content and W/D ratio in lung tissue were significantly reduced compared with those in ALI group (P <0.05).The total and differential inflammatory cells were also significantly reduced in ghrelin therapy group which compared with ALI group .The level of PaO 2 of ghrelin therapy group was increased significantly compared with ALI group (P<0.01), the level of PaCO2 decreased significantly compared with ALI group ( P <0.05 ), and the value of pH was significantly higher compared with ALI group (P<0.05).Pathological examination showed that the lung injuries of ghrelin therapy group were significantly reduced compared with those of ALI group .Conclusion Ghrelin shows a marked reductive effect on the capillary permeability , reduce the injury of lung tissue and exert protective effects on lung in ALI rats .