中国急救医学
中國急救醫學
중국급구의학
CHINESE JOURNAL OF CRITICAL CARE MEDICINE
2014年
10期
927-931
,共5页
肖盐%林兆奋%管军%刁孟元%张浙
肖鹽%林兆奮%管軍%刁孟元%張浙
초염%림조강%관군%조맹원%장절
心脏骤停( CA)%心肺脑复苏%动物模型%兔%神经元特异性烯醇化酶( NSE)%神经功能评分( NDS)
心髒驟停( CA)%心肺腦複囌%動物模型%兔%神經元特異性烯醇化酶( NSE)%神經功能評分( NDS)
심장취정( CA)%심폐뇌복소%동물모형%토%신경원특이성희순화매( NSE)%신경공능평분( NDS)
Cardiac arrest ( CA)%Cardiopulmonary cerebral resuscitation%Animal model%Rabbit%Specificity enolization of neurons ( NSE)%Neural deficit scores ( NDS)
目的:建立一种简单、有效、稳定的家兔电刺激致颤心脏骤停( CA)后脑复苏模型。方法选用20只新西兰雄性家兔,采用体表胸壁及心内膜下交流电诱发室颤的方法制作CA模型,CA 4 min后开始心肺复苏(CPR)。记录手术操作时间(TOP)、自主循环恢复时间(TROSC)、恢复率,除颤、肾上腺素次数,24 h、72 h存活率以及进行72 h大脑皮质凋亡细胞检测。另取10只兔为对照组,只进行致颤前手术操作。结果①20只家兔全部诱发CA成功,室颤发生率为100%。自主循环恢复率为80%;24 h、72 h存活率为50%、30%;除颤和肾上腺素次数为1.20±0.89、1.60±0.75;TOP和TROSC分别为(79±25)min、(268±33)s。②血浆神经元特异性烯醇化酶(NSE)水平显著升高,ROSC 72 h略有回降,但仍高于造模前(P<0.05)。③与对照组比较,ROSC后72 h大脑皮质区出现了较多神经细胞的凋亡(P<0.05)。④神经功能评分(NDS)在复苏后3 h时最低,以后渐趋好转。结论该模型操作简单、结果稳定、复苏成功率高,是一个较为理想的心肺脑复苏研究模型。
目的:建立一種簡單、有效、穩定的傢兔電刺激緻顫心髒驟停( CA)後腦複囌模型。方法選用20隻新西蘭雄性傢兔,採用體錶胸壁及心內膜下交流電誘髮室顫的方法製作CA模型,CA 4 min後開始心肺複囌(CPR)。記錄手術操作時間(TOP)、自主循環恢複時間(TROSC)、恢複率,除顫、腎上腺素次數,24 h、72 h存活率以及進行72 h大腦皮質凋亡細胞檢測。另取10隻兔為對照組,隻進行緻顫前手術操作。結果①20隻傢兔全部誘髮CA成功,室顫髮生率為100%。自主循環恢複率為80%;24 h、72 h存活率為50%、30%;除顫和腎上腺素次數為1.20±0.89、1.60±0.75;TOP和TROSC分彆為(79±25)min、(268±33)s。②血漿神經元特異性烯醇化酶(NSE)水平顯著升高,ROSC 72 h略有迴降,但仍高于造模前(P<0.05)。③與對照組比較,ROSC後72 h大腦皮質區齣現瞭較多神經細胞的凋亡(P<0.05)。④神經功能評分(NDS)在複囌後3 h時最低,以後漸趨好轉。結論該模型操作簡單、結果穩定、複囌成功率高,是一箇較為理想的心肺腦複囌研究模型。
목적:건립일충간단、유효、은정적가토전자격치전심장취정( CA)후뇌복소모형。방법선용20지신서란웅성가토,채용체표흉벽급심내막하교류전유발실전적방법제작CA모형,CA 4 min후개시심폐복소(CPR)。기록수술조작시간(TOP)、자주순배회복시간(TROSC)、회복솔,제전、신상선소차수,24 h、72 h존활솔이급진행72 h대뇌피질조망세포검측。령취10지토위대조조,지진행치전전수술조작。결과①20지가토전부유발CA성공,실전발생솔위100%。자주순배회복솔위80%;24 h、72 h존활솔위50%、30%;제전화신상선소차수위1.20±0.89、1.60±0.75;TOP화TROSC분별위(79±25)min、(268±33)s。②혈장신경원특이성희순화매(NSE)수평현저승고,ROSC 72 h략유회강,단잉고우조모전(P<0.05)。③여대조조비교,ROSC후72 h대뇌피질구출현료교다신경세포적조망(P<0.05)。④신경공능평분(NDS)재복소후3 h시최저,이후점추호전。결론해모형조작간단、결과은정、복소성공솔고,시일개교위이상적심폐뇌복소연구모형。
Objective To establish a simple, effective and stable rabbit brain recovery method on electrically induced fibrillation cardiac arrest (CA).Methods Twenty New Zealand male rabbits were used to set up a rabbit ventricular fibrillation induced CA model by introducing alternating current on the surface under the chest wall and endocardium and then start cardiopulmonary resuscitation (CPR) 4 minutes after CA .The operation time before CPR , restoration of spontaneous circulation ( ROSC ) time, spontaneous circulation response rates , 24 h, 72 h survival rate of the 20 rabbits, the defibrillation frequency , the administration frequency , TOP and TROSC and the apoptotic cells of cerebral cortex on ROSC 72 h have been recorded .For comparison, 10 other rabbits were taken for the surgical operation without fibrillation or CPR .Results ①Cardiac arrest was successfully induced in all 20 rabbits. Incidence of ventricular fibrillation was 100% after electrical stimulation .ROSC rate was 80% and 24 h, 72 h survival rate was 50% (10/20) and 30% (6/20), respectively.The defibrillation frequency was 1.20 ±0.89.The frequency of intravenous injection of adrenaline was 1.60 ±0.75.TOP and TROSC were ( 79 ±25 ) minutes and ( 268 ±33 ) seconds , respectively .②The plasma enzyme specificity enolization of neurons ( NSE) levels increased significantly after building the model .It fell back slightly within 72 hours, but still higher than the level before the model was built (P<0.05).③Apoptosis of nerve cells appeared more in brain cortex after ROSC 72 h than that in the control group (P<0.05).④The lowest NDS was at 3 hours after ROSC , while the NDS increased gradually .The NDS of ROSC 72 h was 72.67 ±9.11 (n=6).Conclusion The rabbit by chest and endocardium induced fibrillation CA model was easy to operate , had stable results and a high recovery rate .It is a relatively ideal cardiopulmonary cerebral resuscitation research model .