中国处方药
中國處方藥
중국처방약
CHINA PRESCRIPTION DRUG
2014年
10期
1-3
,共3页
Nesprin-1%细胞骨架%微管组织中心%细胞迁移
Nesprin-1%細胞骨架%微管組織中心%細胞遷移
Nesprin-1%세포골가%미관조직중심%세포천이
Nesprin-1%Cytoskeleton%MTOC%Cell migration
目的：探索Nesprin-1在连接细胞骨架与细胞核中的作用及对细胞迁移的影响。方法通过基因敲除的方法建立Nesprin-1缺失MEFs细胞，并通过PCR及Western blot进行验证；细胞计数实验检测敲除Nesprin-1对细胞生长的影响；细胞免疫荧光分析细胞骨架蛋白结构及微管组织中心（MTOC）定位；划痕实验用来检测细胞迁移情况。结果Nesprin-1缺失MEFs细胞株建立成功，其生长曲线与野生型细胞一致，但细胞骨架蛋白F-actin丝状结构消失，微管蛋白表达减少，MTOC与细胞核分离，且细胞迁移能力降低。结论Nesprin-1通过连接核骨架和细胞骨架调控细胞骨架硬度及MTOC极化，从而影响细胞迁移。
目的：探索Nesprin-1在連接細胞骨架與細胞覈中的作用及對細胞遷移的影響。方法通過基因敲除的方法建立Nesprin-1缺失MEFs細胞，併通過PCR及Western blot進行驗證；細胞計數實驗檢測敲除Nesprin-1對細胞生長的影響；細胞免疫熒光分析細胞骨架蛋白結構及微管組織中心（MTOC）定位；劃痕實驗用來檢測細胞遷移情況。結果Nesprin-1缺失MEFs細胞株建立成功，其生長麯線與野生型細胞一緻，但細胞骨架蛋白F-actin絲狀結構消失，微管蛋白錶達減少，MTOC與細胞覈分離，且細胞遷移能力降低。結論Nesprin-1通過連接覈骨架和細胞骨架調控細胞骨架硬度及MTOC極化，從而影響細胞遷移。
목적：탐색Nesprin-1재련접세포골가여세포핵중적작용급대세포천이적영향。방법통과기인고제적방법건립Nesprin-1결실MEFs세포，병통과PCR급Western blot진행험증；세포계수실험검측고제Nesprin-1대세포생장적영향；세포면역형광분석세포골가단백결구급미관조직중심（MTOC）정위；화흔실험용래검측세포천이정황。결과Nesprin-1결실MEFs세포주건립성공，기생장곡선여야생형세포일치，단세포골가단백F-actin사상결구소실，미관단백표체감소，MTOC여세포핵분리，차세포천이능력강저。결론Nesprin-1통과련접핵골가화세포골가조공세포골가경도급MTOC겁화，종이영향세포천이。
Objective This study aimed to investigate the role of Nesprin-1 in connection of nucleus and cytoskeleton and controlling cell migration. Methods We established Nesprin-1 deficient MEF cells by knockout mouse model and confirmed by PCR and Western blot. The cell viability was detected by cell counting and growth curve plotting. The position of MTOC and organization of cytoskeleton was performed by immunocytofluorescence. And the wound-healing experiment was product to observe cell migration. Results We had successfully established Nesprin-1 null MEFs and its viability wasn’t affected. However,the organization of F-actin and microtubule was disturbed and MTOC was separated from nucleus which eventually inhibited cell migration. Conclusion Nesprin-1 regulated the stiffness of cytoskeleton and polarization of MTOC by the connection of nucleus and cytoskeleton to affect cell migration.
