医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2014年
10期
48-49
,共2页
克感利咽口服液%挥发油%提取工艺%薄荷脑%胡薄荷酮%薄层鉴别%高效液相色谱法
剋感利嚥口服液%揮髮油%提取工藝%薄荷腦%鬍薄荷酮%薄層鑒彆%高效液相色譜法
극감리인구복액%휘발유%제취공예%박하뇌%호박하동%박층감별%고효액상색보법
Kegan Liyan Oral Liquid%volatile oil%extraction process%menthol%Pulegone%TLC identification%HPLC determination
目的：优化克感利咽口服液挥发油提取工艺，并对挥发油进行薄层鉴别和高效液相含量测定。方法采用水蒸气蒸馏法提取挥发油，以挥发油收得率和挥发油中胡薄荷酮含量的综合得分为考察指标，选择提取前浸泡温度、浸泡时间及提取时间为考察因素，运用正交试验优选工艺条件；采用薄层色谱法对挥发油中薄荷脑进行定性鉴别，高效液相色谱法对挥发油中胡薄荷酮进行定量测定。结果优化的工艺条件是浸泡温度60℃、浸泡时间60分钟、提取2.5小时；薄层色谱鉴定薄荷脑特征性强；色谱条件：色谱柱：Phenomenex Luna（250mm×4.6mm×5μm），流动相：甲醇：水(70∶30)，流速：1ml/min，柱温：35℃，检测波长为252nm，胡薄荷酮在2.9212～186.955μg之间呈良好的线性关系，回收率为99.10%。结论提取工艺合理、可行；建立的薄层鉴别方法及HPLC定量方法准确、简便。
目的：優化剋感利嚥口服液揮髮油提取工藝，併對揮髮油進行薄層鑒彆和高效液相含量測定。方法採用水蒸氣蒸餾法提取揮髮油，以揮髮油收得率和揮髮油中鬍薄荷酮含量的綜閤得分為攷察指標，選擇提取前浸泡溫度、浸泡時間及提取時間為攷察因素，運用正交試驗優選工藝條件；採用薄層色譜法對揮髮油中薄荷腦進行定性鑒彆，高效液相色譜法對揮髮油中鬍薄荷酮進行定量測定。結果優化的工藝條件是浸泡溫度60℃、浸泡時間60分鐘、提取2.5小時；薄層色譜鑒定薄荷腦特徵性彊；色譜條件：色譜柱：Phenomenex Luna（250mm×4.6mm×5μm），流動相：甲醇：水(70∶30)，流速：1ml/min，柱溫：35℃，檢測波長為252nm，鬍薄荷酮在2.9212～186.955μg之間呈良好的線性關繫，迴收率為99.10%。結論提取工藝閤理、可行；建立的薄層鑒彆方法及HPLC定量方法準確、簡便。
목적：우화극감리인구복액휘발유제취공예，병대휘발유진행박층감별화고효액상함량측정。방법채용수증기증류법제취휘발유，이휘발유수득솔화휘발유중호박하동함량적종합득분위고찰지표，선택제취전침포온도、침포시간급제취시간위고찰인소，운용정교시험우선공예조건；채용박층색보법대휘발유중박하뇌진행정성감별，고효액상색보법대휘발유중호박하동진행정량측정。결과우화적공예조건시침포온도60℃、침포시간60분종、제취2.5소시；박층색보감정박하뇌특정성강；색보조건：색보주：Phenomenex Luna（250mm×4.6mm×5μm），류동상：갑순：수(70∶30)，류속：1ml/min，주온：35℃，검측파장위252nm，호박하동재2.9212～186.955μg지간정량호적선성관계，회수솔위99.10%。결론제취공예합리、가행；건립적박층감별방법급HPLC정량방법준학、간편。
Objective To optimize the extraction technology of volatile oil of Kegan Liyan Oral Liquid,and then qualitative and quantitative analysis of volatile oil were measured by thin layer chromatography ( TLC) and high performance thin layer chromatography ( HPLC) respectively. Methods steam distilation were used in the experiment,which was performed with volatile oil yield and pulegone content as the index and the soaking temperature,the soaking time,the time of immersion as affecting factors,then optimizing process condition by orthogonal test.The qualitative identification of menthol was performed by TLC,and the quantitative determination of Pulegone by HPLC.Results Optimal conditions were soak temperature of 60 ℃, soak time of 60 minutes, extraction time of 2.5 hours; identification of menthol by TLC was characteristic strong;the separation was performed on Phenomenex Luna(250mm×4.6mm×5μm). The mobile phase consisted of methanol-water (70:30) at the flow rate of 1mL/min.The column temperature was 35℃ and wavelength was set at 252nm. there was a good linear relationship in the scope of 2.9212~186.955μg for pulegone , The recovery was 99.10%. Conclusions: The extraction technology is reasonable and feasible ,and the developed TLC and HPLC methods are accurate and simple.
