CAJ | 학술논문

目的：观察色素上皮衍生因子（ PEDF ）和转化生长因子（ TGF-β1）在2型糖尿病肾病患者血清中的表达水平，研究PEDF和TGF-β1在糖尿病肾病（ DN）发生发展中的作用，为早期诊断糖尿病肾病提供更敏感的临床检测指标。方法研究对象共150例，分为健康对照组、2型糖尿病组，糖尿病肾病Ⅲ期、Ⅳ期、Ⅴ期患者，分别检测血清PEDF及TGF-β1，静脉血糖（FPG），空腹血糖（FPG）、三酰甘油（TG）、总胆固醇（TC）、血肌酐（sCr）、尿素氮（BUN）、24 h尿量、24 h尿白蛋白、尿肌酐，并计算尿白蛋白排泄率（ UAER ）。结果健康对照组、糖尿病组、糖尿病肾病Ⅲ期组、Ⅳ期组、Ⅴ期组患者血清中PEDF水平、TGF-β1水平依次增高，且组间差异有统计学意义（ P ＜0．05）。结论2型糖尿病肾病患者血清PEDF 和TGF-β1水平显著升高，并且随着病情的进展逐渐增高，两者联合检测为糖尿病肾病的早期诊断和评价治疗效果提供更加敏感、准确、可靠的指标。
목적：관찰색소상피연생인자（ PEDF ）화전화생장인자（ TGF-β1）재2형당뇨병신병환자혈청중적표체수평，연구PEDF화TGF-β1재당뇨병신병（ DN）발생발전중적작용，위조기진단당뇨병신병제공경민감적림상검측지표。방법연구대상공150례，분위건강대조조、2형당뇨병조，당뇨병신병Ⅲ기、Ⅳ기、Ⅴ기환자，분별검측혈청PEDF급TGF-β1，정맥혈당（FPG），공복혈당（FPG）、삼선감유（TG）、총담고순（TC）、혈기항（sCr）、뇨소담（BUN）、24 h뇨량、24 h뇨백단백、뇨기항，병계산뇨백단백배설솔（ UAER ）。결과건강대조조、당뇨병조、당뇨병신병Ⅲ기조、Ⅳ기조、Ⅴ기조환자혈청중PEDF수평、TGF-β1수평의차증고，차조간차이유통계학의의（ P ＜0．05）。결론2형당뇨병신병환자혈청PEDF 화TGF-β1수평현저승고，병차수착병정적진전축점증고，량자연합검측위당뇨병신병적조기진단화평개치료효과제공경가민감、준학、가고적지표。
Objcetive To observe the expression levels of pigment epithelium -derived factor (PEDF ) and transforming growth factor (TGF-β1) in patients with diabetic nephropathy (DN),and to investigate the role of PEDF and TGF-β1 in the pathogenesis and development of diabetic nephropathy so as to provide more susceptive clinical detection index for early diagnosis of diabetic nephropathy .Mtehods One hundred and twenty patients with DN were divided into type 2 diabetes group,DNⅢstage group,DN Ⅳ stage group,DN Ⅴ stage group,with 30 patients in each group,and the other 30 healthy subjects were enrolled as control group .The serum levels of PEDF and TGF-β1,FPG,FPG,TG,TC,sCr,BUN,24h urine volume,24h urinary albumin,urine creatinine were detected by ELISA ,and UAER was calculated .Results The serum levels of PEDF and TGF-β1 in control group,type 2 diabetes group,DNⅢstage group,DNⅣstage group,DNⅤstage group were increased in turn,moreover,there were significant differences among groups ( P <0.05).Conclusio n The serum levels of PEDF and TGF-β1 are obviously increased in patients with DN in early stage ,moreover ,which increase with the progress of pathogenetic condition .The combined detection of PEDF and TGF-β1 provides more susceptive ,accurate,reliable indexes for early diagnosis of diabetic nephropathy .