CAJ | 학술논문

目的：构建真核表达载体 PEGFP-N1-IL-2，为其导入真核或原核细胞打下基础。方法颈脱位处死C57BL／6小鼠，无菌摘除脾脏提取总 RNA，经过 RT-PCR 获取IL-2DNA ，与质粒 PEGFP-N1连接，构建真核表达载体PEGFP-N1-IL-2。结果成功提取了C57BL／6小鼠脾组织总RNA，，经过RT -PCR后获取IL-2DNA并成功构建了真核表达载体PEGFP-N1-IL-2，测序结果与 Genebank 中小鼠 IL-2cDNA （ K02292）序列完全一致。结论真核表达载体PEGFP-N1-IL-2的成功构建，为进一步研究其在原核或真核细胞中表达做好准备。
목적：구건진핵표체재체 PEGFP-N1-IL-2，위기도입진핵혹원핵세포타하기출。방법경탈위처사C57BL／6소서，무균적제비장제취총 RNA，경과 RT-PCR 획취IL-2DNA ，여질립 PEGFP-N1련접，구건진핵표체재체PEGFP-N1-IL-2。결과성공제취료C57BL／6소서비조직총RNA，，경과RT -PCR후획취IL-2DNA병성공구건료진핵표체재체PEGFP-N1-IL-2，측서결과여 Genebank 중소서 IL-2cDNA （ K02292）서렬완전일치。결론진핵표체재체PEGFP-N1-IL-2적성공구건，위진일보연구기재원핵혹진핵세포중표체주호준비。
Objective To establish the fusion plasmid P EGFP-N1-IL-2 in order to provide basis for importing it into eukaryocyte or prokaryocyte.Methods Total RNA was extracted from the spleen of C57BL/6 mice by using TRIzol reagent, then IL-2 DNA was obtained by RT-PCR,and IL-2 DNA was cloned into PEGFP-N1 vectors,finally the fusion plasmid P EGFP-N1-IL-2 was constructed .Results The total RNA was successfully extracted from the spleen of C 57BL /6 mice,IL-2 DNA was obtained by RT-PCR,and eukaryotic expression vector -PEGFP-N1-IL-2 was successfully constructed ,the gene sequencing results showed that the sequence was completely consistent with the Genebank IL -2cDNA sequence (K02292).Conclusion The successful establishment of infusion plasmid P EGFP-N1-IL-2 provides basis for studying its expression in eukaryocyte or prokaryocyte .