CAJ | 학술논문

目的探讨脱细胞大鼠坐骨神经的组织相容性。方法应用低渗脱细胞方法制备脱细胞同种异体神经移植物-脱细胞大鼠坐骨神经(Acel ular Rat Sciatic Nerve,ARSN)，进行组织学观察和免疫组织化学方法检测，肌肉埋植术后分别于第4d和第7d进行其组织相容性分析。结果①ARSN保存了雪旺细胞基底膜管以及神经外膜和束膜的细胞外基质结构，清除了轴突、髓鞘及雪旺细胞神经外膜和束膜的细胞结构。于肌肉埋植后第4d，ARSN神经外膜结构吸收明显，周围出现由新生肉芽组织构成的环状包绕；第7d，ARSN神经外膜结构已被吸收，周围肉芽组织趋于成熟，无淋巴细胞。③免疫组织化学法检测FN和LN可见ARSN基底膜管壁分别呈棕黄色和黄色，但较NRSN阳性略弱。结论采用低渗脱细胞方法制备的周围神经细胞外基质支架保留了对神经再生具有促进作用-FN和LN，具有良好的组织相容性，可随着时间的推移逐渐降解。
목적탐토탈세포대서좌골신경적조직상용성。방법응용저삼탈세포방법제비탈세포동충이체신경이식물-탈세포대서좌골신경(Acel ular Rat Sciatic Nerve,ARSN)，진행조직학관찰화면역조직화학방법검측，기육매식술후분별우제4d화제7d진행기조직상용성분석。결과①ARSN보존료설왕세포기저막관이급신경외막화속막적세포외기질결구，청제료축돌、수초급설왕세포신경외막화속막적세포결구。우기육매식후제4d，ARSN신경외막결구흡수명현，주위출현유신생육아조직구성적배상포요；제7d，ARSN신경외막결구이피흡수，주위육아조직추우성숙，무림파세포。③면역조직화학법검측FN화LN가견ARSN기저막관벽분별정종황색화황색，단교NRSN양성략약。결론채용저삼탈세포방법제비적주위신경세포외기질지가보류료대신경재생구유촉진작용-FN화LN，구유량호적조직상용성，가수착시간적추이축점강해。
Objective To explore histocompatibility of acellular rat sciatic nerve. Methods Prepared acellular rat sciatic nerve(ARSN) with hypotonic acellular treatment. Histological observation and examined by immunohischemical method. Ananlyzed histocompatibility after implantation in muscle respectively. Results ①ARSN reserved basement membrane tube of SC (Schwann cells) and ECM (extracellular matrix) of epineurium and perineurium, while axis-cylinder, myelin sheath and cellstructure of epineurium and perineurium were cleared away. ②Histological observation of ARSN after implantation in muscle. After HE staining showed: on the 4th day, epineurium left in ARSN group was absorbed obviously. Also, a ring structure formed of new-born granulation tissue surrounded ARSN. On 7th day of implantation, epineurium left in ARSN was absorbed completely and granulation tissue tended to be mature. And we saw no lymphocyte. ③Examination of laminin (LN) and fibronectin (FN) by immunohistochemical method, We could see that basement membrane was yel ow or brown showing positive reaction. Conclusion The cytoskeletal of acl ular peripheral ECM we prepared reserved important components of ECM of nerve tissue which might promote nerve regeneration, LN and FN. They had good histocompatibility and would be degraded with time flies.