CAJ | 학술논문

水稻黑条矮缩病毒（Rice black-streaked dwarf virus，RBSDV）是斐济病毒属的成员，由昆虫介体灰飞虱传播，能够在植物宿主和昆虫介体中复制。同其他斐济病毒成员一样，RBSDV的复制与装配是在细胞质病毒包涵体结构即毒质结构里进行的。 RBSDV有10条基因组dsRNAs（S1-S10），其第9条片段（S9）的第一个阅读框（ORF）编码的蛋白P9-1是形成毒质结构的框架蛋白。将P9-1与绿色荧光蛋白（GFP）融合后，在本氏烟草的表皮细胞中单独表达，通过激光共聚焦扫描显微镜观察发现P9-1可以形成包涵体结构；利用双分子荧光互补（BiFC）实验证明了P9-1具有自我互作的能力，并形成包涵体结构。
수도흑조왜축병독（Rice black-streaked dwarf virus，RBSDV）시비제병독속적성원，유곤충개체회비슬전파，능구재식물숙주화곤충개체중복제。동기타비제병독성원일양，RBSDV적복제여장배시재세포질병독포함체결구즉독질결구리진행적。 RBSDV유10조기인조dsRNAs（S1-S10），기제9조편단（S9）적제일개열독광（ORF）편마적단백P9-1시형성독질결구적광가단백。장P9-1여록색형광단백（GFP）융합후，재본씨연초적표피세포중단독표체，통과격광공취초소묘현미경관찰발현P9-1가이형성포함체결구；이용쌍분자형광호보（BiFC）실험증명료P9-1구유자아호작적능력，병형성포함체결구。
Rice black-streaked dwarf virus (RBSDV), a member of the genus Fijivirus, is transmitted in nature by the small brown planthopper (Laodelphax striatellus) and can replicate in cells of both host plants and insect vector . RBSDV, like other members of the family Reovirus , is thought to replicate and assemble within cytoplasmic viral inclusion bodies, commonly called viroplasms.It has genomic segments of 10 dsRNAs (S1-S10) and P9-1 encoded by the first open reading frame in the ninth segment ( S9) of the genomic RNAs is an essential protein for the formation of viroplasms.Fused with green fluorescent protein (GFP), P9-1 was able to form inclusion bodies when expressed alone in Nicotiana benthamiana epidermal cell.Bimolecular fluorescence complementation (BiFC) assay suggested P9-1 had the ability of self-interaction and formed inclusion bodies .