CAJ | 학술논문

目的：探讨甘肃省武威地区胃癌患者EB病毒（Epstein-Barr virus，EBV）感染状况及miR-101、EZH2、COX-2在EBV相关胃癌发生发展中的作用。方法：应用组织芯片、原位杂交和免疫组织化学技术检测120例胃癌组织及相应癌旁组织中EBV小RNA（EBER）、miR-101、EZH2、COX-2的表达情况。结果：120例胃癌组织EBV阳性率为10.0%，EBV相关胃癌有较少的淋巴结转移，好发于贲门、胃体（P<0.05）。miR-101、EZH2、COX-2在120例胃癌组织和相应癌旁组织的阳性率差异有统计学意义（P<0.05）。12例EBV阳性胃癌组织miR-101、EZH2、COX-2和108例EBV阴性胃癌组织3个分子的表达率差异有统计学意义（P<0.05）。胃癌组织EBV感染和miR-101表达呈正相关，EBV相关胃癌组织miR-101表达和淋巴结转移、COX-2、EZH2表达均呈负相关（P<0.05）。结论：EB病毒感染与武威地区胃癌的发生有一定关系；EBV相关胃癌和EBV阴性胃癌在淋巴结转移和发生部位的差异有统计学意义；miR-101、EZH2、COX-2与EB病毒相关胃癌的发展有一定关系。
목적：탐토감숙성무위지구위암환자EB병독（Epstein-Barr virus，EBV）감염상황급miR-101、EZH2、COX-2재EBV상관위암발생발전중적작용。방법：응용조직심편、원위잡교화면역조직화학기술검측120례위암조직급상응암방조직중EBV소RNA（EBER）、miR-101、EZH2、COX-2적표체정황。결과：120례위암조직EBV양성솔위10.0%，EBV상관위암유교소적림파결전이，호발우분문、위체（P<0.05）。miR-101、EZH2、COX-2재120례위암조직화상응암방조직적양성솔차이유통계학의의（P<0.05）。12례EBV양성위암조직miR-101、EZH2、COX-2화108례EBV음성위암조직3개분자적표체솔차이유통계학의의（P<0.05）。위암조직EBV감염화miR-101표체정정상관，EBV상관위암조직miR-101표체화림파결전이、COX-2、EZH2표체균정부상관（P<0.05）。결론：EB병독감염여무위지구위암적발생유일정관계；EBV상관위암화EBV음성위암재림파결전이화발생부위적차이유통계학의의；miR-101、EZH2、COX-2여EB병독상관위암적발전유일정관계。
Objective:This study was conducted to investigate Epstein-Barr virus (EBV) infection in gastric cancer in Wuwei ar-ea of Gansu province (China) and the roles of miR-101, EZH2, and COX-2 in EBV-associated gastric carcinomas (EBVaGC). Meth-ods:Tissue microarray technique, immunohistochemistry, and in situ hybridization were performed to detect the expression of EBV-en-coded small RNAs (EBERs), miR-101, EZH2, and COX-2, in gastric cancer tissues (n=120) and the corresponding adjacent tissues (n=120). Results:The positive rate of EBV was 10.0% in 120 cases of gastric cancer tissues. EBVaGC was not significantly associated with lymph node metastasis and developed most often in the cardia and body (P<0.05). The differences in the positive rates of miR-101, EZH2, and COX-2 in 120 cases of gastric cancer tissues and corresponding adjacent tissues were significant (P<0.05). The differences in the positive rates of miR-101, EZH2, and COX-2 in 12 cases of EBVaGC and in 108 cases of EBV-negative gastric can-cer (EBVnGC) tissues were significant (P<0.05). The infection of EBV in gastric cancer tissues was positively related to miR-101 ex-pression. By contrast, miR-101 expression was negatively related to lymph node metastasis and expression of COX-2 and EZH2 in EB-VaGC tissues (P<0.05). Conclusion:EBV infection was related to gastric cancer in Wuwei area of Gansu province. EBVaGC and EB-VnGC have significant differences in lymph node metastasis and in the location of cancer. MiR-101, EZH2, and COX-2 were related to the development of EBVaGC.