CAJ | 학술논문

　　在摇瓶液体培养条件下，优化冬虫夏草液体种子培养条件，采用单因素和正交试验法，研究了不同培养条件对冬虫夏草液体细胞生长的影响。结果表明，在所考察的因素中，冬虫夏草液体种子最优培养条件为：培养温度24℃，转速150 r/min，装液量30%（75 mL/250 mL）。在此条件下，经摇瓶培养，获得最大菌体干重为10.45 g/L。
　　재요병액체배양조건하，우화동충하초액체충자배양조건，채용단인소화정교시험법，연구료불동배양조건대동충하초액체세포생장적영향。결과표명，재소고찰적인소중，동충하초액체충자최우배양조건위：배양온도24℃，전속150 r/min，장액량30%（75 mL/250 mL）。재차조건하，경요병배양，획득최대균체간중위10.45 g/L。
At the submerged cultivation conditions in the shake flask , to optimize the cultivation conditions for Inoculums of Cordyceps sinensis,single factor and orthogonal array design were employed for investigating the effects of different cultivation conditions on cell growth of Cordyceps sinensis. The result indicated that the optimum cultivation conditions for Cordyceps sinensis were as follows:cultivation temperature 24 ℃,rotation speed 150 r/min, the medium volume in the flask 30%(75 mL/250 mL). At the cultivation conditions, we get 10.45 g DW/L at the submerged cultivation in the shake flask.