CAJ | 학술논문

　　研究了高效液相色谱测定月饼中丙烯酰胺的方法。采用Inertsil ODS-SP（250 mm×46 mm）色谱柱，以210 nm为检测波长，以5%乙腈水溶液作为流动相，流速为0.8 mL/min，柱温25℃，进样量20滋L。该方法的最低检测限为0.124滋g/kg。丙烯酰胺在0.1 mg/L~1.0 mg/L范围内呈良好的线性关系，回收率大于80%。结果显示：固相萃取与溶剂萃取都适合作为样品预处理方法，适合这类焙烤食品的检测。
　　연구료고효액상색보측정월병중병희선알적방법。채용Inertsil ODS-SP（250 mm×46 mm）색보주，이210 nm위검측파장，이5%을정수용액작위류동상，류속위0.8 mL/min，주온25℃，진양량20자L。해방법적최저검측한위0.124자g/kg。병희선알재0.1 mg/L~1.0 mg/L범위내정량호적선성관계，회수솔대우80%。결과현시：고상췌취여용제췌취도괄합작위양품예처리방법，괄합저류배고식품적검측。
The method of high performance liquid chromatography for acrylamide determination in moon cake was established. The acrylamide was separated on a C18 column (Inertsil ODS-SP,250 mm×46 mm) with 5%acetonitrile-water solution as mobile phase, and detected at 210 nm. The limit of detection is 0.124μg/kg. The linear plots were obtained were obtained between 0.1 mg/L-1.0 mg/L. Overall recoveries were higher than 80%and RSD value were between 0.17%and 4.33%. The experiment results showed that solid phase extraction and solvent extraction were suitable for analysis of acrylamide in moon cake.