食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2013年
13期
18-22
,共5页
鸭血%超氧化物歧化酶%热变性%响应面法
鴨血%超氧化物歧化酶%熱變性%響應麵法
압혈%초양화물기화매%열변성%향응면법
duck blood%superoxide dismutase(SOD)%heating denaturation method%response surface method (RSM)
以新鲜鸭血为原料,研究鸭血超氧化物歧化酶(SOD)在热变性环境下的提取工艺。通过单因素试验确定了热变性温度、热变性时间、硫酸锌浓度3个因素的取值范围,并根据中心组合设计原理及响应面分析得出最优提取条件。结果表明:热变性温度55.3℃,热变性时间12 min,硫酸锌的质量浓度3.9%,硫酸铜的质量浓度1%,SOD比活为1224.37 U/mg。
以新鮮鴨血為原料,研究鴨血超氧化物歧化酶(SOD)在熱變性環境下的提取工藝。通過單因素試驗確定瞭熱變性溫度、熱變性時間、硫痠鋅濃度3箇因素的取值範圍,併根據中心組閤設計原理及響應麵分析得齣最優提取條件。結果錶明:熱變性溫度55.3℃,熱變性時間12 min,硫痠鋅的質量濃度3.9%,硫痠銅的質量濃度1%,SOD比活為1224.37 U/mg。
이신선압혈위원료,연구압혈초양화물기화매(SOD)재열변성배경하적제취공예。통과단인소시험학정료열변성온도、열변성시간、류산자농도3개인소적취치범위,병근거중심조합설계원리급향응면분석득출최우제취조건。결과표명:열변성온도55.3℃,열변성시간12 min,류산자적질량농도3.9%,류산동적질량농도1%,SOD비활위1224.37 U/mg。
With fresh duck blood as raw materials,study of superoxide dismutase (SOD) from duck blood in thermal denaturation conditions extraction process.hrough single factor experiment to determine the thermal denaturation temperature, heating time, the zinc sulfate concentration by a factor of three range, and according to a central composite design principle and response surface analysis the optimum extraction conditions.The results show that:the thermal denaturation temperature at 55.3℃, heating time 12 min,the concentration of 3.9%zinc sulfate, copper sulfate concentration of 1%, SOD specific activity of 1 224.37 U/mg.