CAJ | 학술논문

　　以核桃蛋白粉为原料制备核桃蛋白抗氧化活性肽，分别采用中性蛋白酶、碱性蛋白酶、木瓜蛋白酶对核桃蛋白进行酶解，测定了不同酶作用下的核桃多肽抗氧化活性，确定碱性蛋白酶是酶解核桃蛋白的最适蛋白酶。研究了酶解温度、酶解时间、酶解pH、底物浓度、酶添加量等酶解条件对酶解产物抗氧化活性的影响。结果表明：不同的酶解条件对酶解产物核桃多肽的抗氧化活性有显著影响，最佳酶解条件为：温度50℃，时间120 min，pH8，底物浓度3%，酶添加量3%，在此酶解条件下制得的核桃多肽对羟基自由基和超氧阴离子的清除率分别为53.8%和50.0%，还原能力为51.7%。
　　이핵도단백분위원료제비핵도단백항양화활성태，분별채용중성단백매、감성단백매、목과단백매대핵도단백진행매해，측정료불동매작용하적핵도다태항양화활성，학정감성단백매시매해핵도단백적최괄단백매。연구료매해온도、매해시간、매해pH、저물농도、매첨가량등매해조건대매해산물항양화활성적영향。결과표명：불동적매해조건대매해산물핵도다태적항양화활성유현저영향，최가매해조건위：온도50℃，시간120 min，pH8，저물농도3%，매첨가량3%，재차매해조건하제득적핵도다태대간기자유기화초양음리자적청제솔분별위53.8%화50.0%，환원능력위51.7%。
Walnut protein powder was used as raw material to prepare walnut polypeptide. Neutral protease , alcalase, papain were used respectively to hydrolyze walnut protein, through determining anti-oxidative activity of walnut polypeptide on different enzymes, the results that alcalase was optimal protease. Effect of temperature, time, pH, substrate concentration, adding amount of enzyme on anti-oxidative activity of enzymolysis product were studied. The results showed that , different hydrolysis conditions on anti-oxidative activity of enzymolysis product have significant effect. The most suitable condition was:temperature 50℃, time 120 min, pH 8, substrate concentration 3%, and adding amount of enzyme 3%. Under these conditions , the scavenging rate of hydroxyl radical and superoxide anion radical of walnut polypeptide was 53.8%and 50.0%respectively, reducibility power was 51.7%.