CAJ | 학술논문

　　芥子酶能分解底物硫代葡萄糖苷产生萝卜硫素等抗癌物质。本研究拟从十字花科植物寄生菜蚜体内分离产芥子酶微生物。通过RSM培养基富集培养，氯化钯-硫苷降解率法初筛，葡萄糖产量法复筛。分离获得9株产芥子酶菌株，其中DS12菌株的酶活最高，为1.486 IU；并且在VC浓度为0.28滋g/uL时，酶活最大；在发酵42 h，生长量最大。DS12可作为生产芥子酶的潜力菌株。
　　개자매능분해저물류대포도당감산생라복류소등항암물질。본연구의종십자화과식물기생채아체내분리산개자매미생물。통과RSM배양기부집배양，록화파-류감강해솔법초사，포도당산량법복사。분리획득9주산개자매균주，기중DS12균주적매활최고，위1.486 IU；병차재VC농도위0.28자g/uL시，매활최대；재발효42 h，생장량최대。DS12가작위생산개자매적잠력균주。
Myrosinase can degrade glucosinolates to produce sulforaphane and other anti-cancer materials. This study aimed to isolate myrosinase producing microorganisms from cabbage aphid from Lipaphis erysimi that specifically parasitizing Brassicaceae plants. Throngh enrichment culture with RSM medium , screening with palladium chloride-the glucosinolate degradation rate and rescreening with glucose production method , 9 strains producing myrosinase were isolated in this paper , in which the D12 strain has the most enzyme amounts up to 1.486 IU, its enzyme activity is most at VC concentration 0.28 μg/μL, its maximum amount of growth arised at fermentation time 42 hours. So DS12 has the potential for producing myrosinase.