CAJ | 학술논문

　　主要探讨辣木茎叶中水溶性多糖的提取工艺条件以及抗氧化活性。以辣木茎叶干粉为原料，采用水为提取剂，通过单因素和正交试验对浸提温度、浸提时间及料液比进行研究；采用水杨酸法和邻苯三酚法分别测定辣木多糖对羟自由基以及超氧阴离子的清除率，以确定提取物的抗氧化活性。实验条件下辣木茎叶多糖最佳提取工艺条件为料液比1∶20（g/mL）、浸提温度80℃、浸提时间120 min，在此条件下，辣木粗多糖的提取率可达5.66%；多糖提取物对羟自由基及超氧阴离子均有清除作用，且随着提取物浓度的提高对二者的清除作用逐渐增强，存在剂量效应关系。清除作用的半数抑制率（IC50）分别是7.2528 mg/mL和2.5011 mg/mL。
　　주요탐토랄목경협중수용성다당적제취공예조건이급항양화활성。이랄목경협간분위원료，채용수위제취제，통과단인소화정교시험대침제온도、침제시간급료액비진행연구；채용수양산법화린분삼분법분별측정랄목다당대간자유기이급초양음리자적청제솔，이학정제취물적항양화활성。실험조건하랄목경협다당최가제취공예조건위료액비1∶20（g/mL）、침제온도80℃、침제시간120 min，재차조건하，랄목조다당적제취솔가체5.66%；다당제취물대간자유기급초양음리자균유청제작용，차수착제취물농도적제고대이자적청제작용축점증강，존재제량효응관계。청제작용적반수억제솔（IC50）분별시7.2528 mg/mL화2.5011 mg/mL。
In order to explore the extraction conditions of water-soluble polysaccharide from the Moringa oleifera leaves and antioxidant activity of it,dry powder was used as raw materials and using water as extracting solvent to extract polysaccharide from Moringa oleifera. The extraction temperature, time and solid-liquid ratio were optimized by orthogonal experiments. Then,use methods of Salicylic acid and Pyrogallol to test antioxidant activity on polysaccharide from Moringa oleifera in different concentrations respectively. The results showed that the extraction rate was up to 5.66%,the optimal extraction conditions was extract 120 min ,using 80℃hot water at a solid-liquid ratio of 1∶20 (g/mL). The polysaccharides from Moringa oleifera leaf had a certain degree of antioxidant activities in vitro:scavenge OH·oand O2-in a dose-dependant manner with the IC50 of 7.252 8 mg/ml and 2.501 1 mg/ml respectively.