CAJ | 학술논문

　　在Lipozyme 435脂肪酶催化下，利用异VC与山茶油、猪油、麻油和菜籽油分别发生酯交换反应，合成异VC混合脂肪酸酯，产物用柱层析法分离纯化。以山茶油与异VC反应为例，详细考察了反应溶剂、反应时间、反应物配比、酶用量和油脂回收循环使用对反应转化率的影响。优化条件下，反应转化率最高达到48%。将合成的异VC山茶油脂肪酸酯、异VC猪油脂肪酸酯、异VC芝麻油脂肪酸酯和异VC菜籽油脂肪酸酯应用于新鲜山茶油中，用过氧化值法（POV）进行抗氧化试验。结果表明，异VC混合脂肪酸酯是良好的脂溶性抗氧化剂，并表现出明显的剂效关系。
　　재Lipozyme 435지방매최화하，이용이VC여산다유、저유、마유화채자유분별발생지교환반응，합성이VC혼합지방산지，산물용주층석법분리순화。이산다유여이VC반응위례，상세고찰료반응용제、반응시간、반응물배비、매용량화유지회수순배사용대반응전화솔적영향。우화조건하，반응전화솔최고체도48%。장합성적이VC산다유지방산지、이VC저유지방산지、이VC지마유지방산지화이VC채자유지방산지응용우신선산다유중，용과양화치법（POV）진행항양화시험。결과표명，이VC혼합지방산지시량호적지용성항양화제，병표현출명현적제효관계。
D-isoascorbyl mixed-fatty acid esters were synthesized with Lipozyme 435 as catalyst by the transesterification of D-isoascorbic acid with camellia oil, lard, sesame oil and canola oil, respectively. The effect of factors such as reaction solvent, reaction time, the ratio of reactants, the amount of lipase, and the recycled oils and fats on the reaction conversion were explored with the synthesis of D-isoascorbyl camellia oil fatty acid esters as reaction model to optimize the reaction conditions. Under the optimized conditions , the reaction conversion could reach 48 %. The antioxidation activities of D-isoascorbyl camellia oil fatty acid esters, D-isoascorbyl lard fatty acid esters, D-isoascorbyl sesame oil fatty acid ester and D-isoascorbyl rapeseed oil fatty acid ester in camellia oil was tested by POV determination , respectively. The results showed that all the synthesized D-isoascorbyl mixed-fatty acid esters were excellent fat-soluble antioxidants and that their antioxidation activities increased with the applied concentration.