CAJ | 학술논문

　　研究中国蜂胶和巴西蜂胶乙醇提取物对过氧化氢（H2O2）诱导的大鼠BRL肝细胞损伤的保护功效。方法：BRL肝细胞经H2O2预孵1 h后，分别经DMEM培养液、中国蜂胶乙醇提取物（EECP）、巴西蜂胶乙醇提取物（EEBP）处理24 h，分别测定细胞存活率、细胞凋亡率以及细胞培养液中谷丙转氨酶（ALT）、谷草转氨酶（AST）、超氧化物歧化酶（SOD）以及丙二醛（MDA）的变化。与H2O2组相比，EECP和EEBP均显著提高H2O2损伤后BRL肝细胞的存活率，抑制细胞的凋亡；降低细胞培养液中ALT和AST水平；提高SOD的活性。尽管中国蜂胶和巴西蜂胶化学组分不同，但中国蜂胶和巴西蜂胶均具有较好的保肝功效，其机制与提高细胞存活率、抑制细胞凋亡以及降低细胞内AST、ALT水平，升高SOD活性有关。
　　연구중국봉효화파서봉효을순제취물대과양화경（H2O2）유도적대서BRL간세포손상적보호공효。방법：BRL간세포경H2O2예부1 h후，분별경DMEM배양액、중국봉효을순제취물（EECP）、파서봉효을순제취물（EEBP）처리24 h，분별측정세포존활솔、세포조망솔이급세포배양액중곡병전안매（ALT）、곡초전안매（AST）、초양화물기화매（SOD）이급병이철（MDA）적변화。여H2O2조상비，EECP화EEBP균현저제고H2O2손상후BRL간세포적존활솔，억제세포적조망；강저세포배양액중ALT화AST수평；제고SOD적활성。진관중국봉효화파서봉효화학조분불동，단중국봉효화파서봉효균구유교호적보간공효，기궤제여제고세포존활솔、억제세포조망이급강저세포내AST、ALT수평，승고SOD활성유관。
To investigate the protective effect of ethanol extract of Chinese propolis (EECP) and ethanol extract of Brazilian propolis (EEBP) on the injury of rat BRL hepatocytes induced by hydrogen peroxide (H2O2) in vitro. BRL hepatocytes were administrated by H2O2 for 1 h, then treated by DMEM culture, EECP and EEBP respectively for 24 h. Cell viability, apoptosis rate and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), super oxygen dehydrogenises (SOD) and malondialdehyde (MDA) in supernate were measured 24 hours later. cell viability of BRL hepatocytes induced by H2O2 was significantly increased by EECP and EEBP for 24 h; the apoptosis rate was obviously depressed. The levels of ALT and AST supernate were depressed by EECP and EEBP, and the SOD activity was increased obviously by EECP and EEBP. Chinese propolis and Brazilian propolis also have good protective effects on the injury of hepatocytes , and the possible mechanisms are related to increasing the cell viability and SOD activity , depressing apoptosis rate and the levels of ALT and AST.