CAJ | 학술논문

　　研究益智果实乙酸乙酯部位的抗氧化能力。采用液液分配法从益智果实乙醇提取物中萃取得到乙酸乙酯部位，用硅胶柱色谱对其进一步分离纯化，得到4个洗脱部分。分别采用DPPH·清除法、ABTS·+清除法、总还原能力以及福林酚方法测定5个提取物的抗氧化能力和总多酚含量。实验结果表明，益智果实乙酸乙酯部位及其各部分均具有较强的抗氧化能力，其中石油醚∶乙酸乙酯（4∶1，体积比）洗脱部分的抗氧化能力最强。各提取物总多酚含量测定结果与其抗氧化能力呈正相关，表明提取物中起抗氧化作用的物质主要为多酚类化合物。
　　연구익지과실을산을지부위적항양화능력。채용액액분배법종익지과실을순제취물중췌취득도을산을지부위，용규효주색보대기진일보분리순화，득도4개세탈부분。분별채용DPPH·청제법、ABTS·+청제법、총환원능력이급복림분방법측정5개제취물적항양화능력화총다분함량。실험결과표명，익지과실을산을지부위급기각부분균구유교강적항양화능력，기중석유미∶을산을지（4∶1，체적비）세탈부분적항양화능력최강。각제취물총다분함량측정결과여기항양화능력정정상관，표명제취물중기항양화작용적물질주요위다분류화합물。
Antioxidant activities of the ethyl acetate fraction of the fruit of Alpinia oxyphylla were investigated in this work. Ethyl acetate fraction was obtained from the ethanol extract of the fruit of Alpinia oxyphylla by using liquid-liquid partition, and then was further separated by silica gel column chromatography to acquire four subfractions. The total phenolic content and antioxidant activity of the ethyl acetate fraction and its four subfractions were determined by Folin-Ciocalteu method and DPPH·, ABTS·+, and reducing power assay. The results showed that the ethyl acetate fraction and its subfractions possessed significant antioxidant effect against free radicals and reducing power, of which the subfraction eluted by petroleum ether:ethyl acetate (4∶1,volume ratio) exhibited the most potent antioxidant activity. Meanwhile the total phenolic content of the fractions was found to be positive correlation to their antioxidant activity , suggesting that the phenolic compounds may be responsible for the antioxidant effect of these fractions.