CAJ | 학술논문

　　目的：应用跨种属策略研究MCM7蛋白在人、大鼠、树鼩3个不同种属肝癌、癌旁组织及正常肝组织中的表达，探讨MCM7与肝癌发生发展的关系及临床意义。方法：应用Western blot及免疫组织化学技术检测MCM7蛋白在人、大鼠、树鼩的肝癌、癌旁组织及正常肝组织中的表达并进行临床病理因素分析。结果：Western blot结果显示，MCM7蛋白在人和大鼠肝癌组织中的表达水平显著高于对应癌旁及正常肝组织（P均<0.05），在树鼩肝癌组织中MCM7蛋白的表达水平亦高于其癌旁组织及正常肝组织，但差异无统计学意义（P均>0.05）。免疫组织化学结果显示，MCM7蛋白主要在细胞核中表达，其在人、大鼠、树鼩肝癌组织中的阳性表达率明显高于癌旁及正常肝组织（P均<0.05）；MCM7蛋白的表达水平与患者肝癌分期、肝外转移及术后复发相关（P均<0.05）。而3种属的癌旁组织与正常肝组织相比，两种技术检测结果显示差异均无统计学意义（P均>0.05）。结论：MCM7蛋白在肝癌发生发展中可能起着关键分子的作用，并与人肝癌的临床分期，肝外转移及术后复发密切相关，有可能成为肝癌治疗的新靶点。
　　목적：응용과충속책략연구MCM7단백재인、대서、수구3개불동충속간암、암방조직급정상간조직중적표체，탐토MCM7여간암발생발전적관계급림상의의。방법：응용Western blot급면역조직화학기술검측MCM7단백재인、대서、수구적간암、암방조직급정상간조직중적표체병진행림상병리인소분석。결과：Western blot결과현시，MCM7단백재인화대서간암조직중적표체수평현저고우대응암방급정상간조직（P균<0.05），재수구간암조직중MCM7단백적표체수평역고우기암방조직급정상간조직，단차이무통계학의의（P균>0.05）。면역조직화학결과현시，MCM7단백주요재세포핵중표체，기재인、대서、수구간암조직중적양성표체솔명현고우암방급정상간조직（P균<0.05）；MCM7단백적표체수평여환자간암분기、간외전이급술후복발상관（P균<0.05）。이3충속적암방조직여정상간조직상비，량충기술검측결과현시차이균무통계학의의（P균>0.05）。결론：MCM7단백재간암발생발전중가능기착관건분자적작용，병여인간암적림상분기，간외전이급술후복발밀절상관，유가능성위간암치료적신파점。
Objective:To test the expression of Minichromosome maintenance complex component 7(MCM7) protein in hepato-cellular carcinoma(HCC) of different species including human, rat and tree shrew (tupaia) by cross-species oncogenomics approach, and to investigate the relationship between the expression of MCM7 and the development of hepatocellular carcinoma and its clinical significance. Methods:Western blot and Immunohistochemistry were applied to detect the expression levels of MCM7 protein in HCC tissues,corresponding HCC-adjacent liver tissues and normal liver tissues collected from different species including human, rat and tree shrew, respectively. The clinicopathologic factors were also analyzed with the results of Immunohistochemistry. Results:Western blot analysis showed that the expression of MCM7 protein in HCC tissues of human and rat were higher than that in corresponding HCC-ad-jacent liver tissues and normal liver tissues, respectively and significantly (P<0.05). However, the expression of MCM7 protein in HCC tissues of tree shrew were also higher than that in corresponding HCC-adjacent liver tissues and normal liver tissues, but no significant difference was found among three types of tissues (P>0.05).There was also no significant difference between HCC-adjacent liver tis-sues and normal liver tissues in three species (P>0.05). Immunohistochemical analysis showed that MCM7 protein was mainly ex-pressed in nucleus of HCC cells, and the positive rate of MCM7 protein in HCC tissues of human, rat and tree shrew were significantly higher than that in corresponding HCC-adjacent liver tissues and normal liver tissues, respectively (P<0.05). However, no significant difference was found between HCC-adjacent liver tissues and normal liver tissues (P>0.05). Moreover, the protein level of MCM7 was intimately related to patient's HCC stage, extrahepatic metastases and postoperative recurrence (P<0.05). Conclusion:MCM7 protein might play a pivotal role in hepatocarcinogenesis. In addition, it was probably related to patient's HCC stage, extrahepatic metastases and postoperative recurrence. It seems very likely that MCM7 may be applied as a new molecular target in HCC prevention and treat-ment.