检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2013年
16期
2102-2103
,共2页
强直性脊柱炎%T淋巴细胞%流式细胞术
彊直性脊柱炎%T淋巴細胞%流式細胞術
강직성척주염%T림파세포%류식세포술
ankylosing spondylitis%T lymphocyte%flow cytometry
目的探讨强直性脊柱炎（AS ）患者外周血CD4＋ CD45RA＋、CD4＋ CD45RO＋、CD8＋ CD45RA＋和CD8＋CD45RO＋ T淋巴细胞亚群的表达及与免疫功能的关系。方法用流式细胞仪检测55例AS患者（分AS病情活动组30例、稳定组25例）CD4＋、CD8＋细胞CD45RO、CD45RA的表达，并与健康对照组进行比较。结果 AS患者活动期CD4＋CD45RO＋、CD8＋CD45RO＋ T淋巴细胞表达明显高于健康对照组（ P＜0．01），CD4＋ CD45RA＋、CD8＋CD45RA＋明显对低于健康对照组（P＜0．01）；稳定期CD4＋ CD45RO＋、CD8＋ CD45RO＋与活动期比较明显降低（ P＜0．05），但仍高于健康对照组（ P＜0．05）；CD4＋ CD45RA＋、CD8＋ CD45RA＋与活动期相比明显升高（ P＜0．05），但仍低于健康对照组（ P＜0．05）。结论 CD4＋ CD45RO＋、CD8＋ CD45RO＋和 CD4＋ CD45RA＋、CD8＋CD45RA＋ T淋巴细胞的异常表达是导致强直性脊柱炎患者机体免疫功能失去平衡的重要原因。
目的探討彊直性脊柱炎（AS ）患者外週血CD4＋ CD45RA＋、CD4＋ CD45RO＋、CD8＋ CD45RA＋和CD8＋CD45RO＋ T淋巴細胞亞群的錶達及與免疫功能的關繫。方法用流式細胞儀檢測55例AS患者（分AS病情活動組30例、穩定組25例）CD4＋、CD8＋細胞CD45RO、CD45RA的錶達，併與健康對照組進行比較。結果 AS患者活動期CD4＋CD45RO＋、CD8＋CD45RO＋ T淋巴細胞錶達明顯高于健康對照組（ P＜0．01），CD4＋ CD45RA＋、CD8＋CD45RA＋明顯對低于健康對照組（P＜0．01）；穩定期CD4＋ CD45RO＋、CD8＋ CD45RO＋與活動期比較明顯降低（ P＜0．05），但仍高于健康對照組（ P＜0．05）；CD4＋ CD45RA＋、CD8＋ CD45RA＋與活動期相比明顯升高（ P＜0．05），但仍低于健康對照組（ P＜0．05）。結論 CD4＋ CD45RO＋、CD8＋ CD45RO＋和 CD4＋ CD45RA＋、CD8＋CD45RA＋ T淋巴細胞的異常錶達是導緻彊直性脊柱炎患者機體免疫功能失去平衡的重要原因。
목적탐토강직성척주염（AS ）환자외주혈CD4＋ CD45RA＋、CD4＋ CD45RO＋、CD8＋ CD45RA＋화CD8＋CD45RO＋ T림파세포아군적표체급여면역공능적관계。방법용류식세포의검측55례AS환자（분AS병정활동조30례、은정조25례）CD4＋、CD8＋세포CD45RO、CD45RA적표체，병여건강대조조진행비교。결과 AS환자활동기CD4＋CD45RO＋、CD8＋CD45RO＋ T림파세포표체명현고우건강대조조（ P＜0．01），CD4＋ CD45RA＋、CD8＋CD45RA＋명현대저우건강대조조（P＜0．01）；은정기CD4＋ CD45RO＋、CD8＋ CD45RO＋여활동기비교명현강저（ P＜0．05），단잉고우건강대조조（ P＜0．05）；CD4＋ CD45RA＋、CD8＋ CD45RA＋여활동기상비명현승고（ P＜0．05），단잉저우건강대조조（ P＜0．05）。결론 CD4＋ CD45RO＋、CD8＋ CD45RO＋화 CD4＋ CD45RA＋、CD8＋CD45RA＋ T림파세포적이상표체시도치강직성척주염환자궤체면역공능실거평형적중요원인。
Objective To discuss on the peripheral blood CD4+ CD45RA+ ,CD4+ CD45RO+ ,CD8+ CD45RA+and CD8+CD45RO+ T lymphocyte subgroup expression and immune function in patients with ankylosing spondylitis (AS) .Methods To detect the expression of CD45RO and CD45RA in CD4+ and CD8+ T lymphocyte by flow cy-tometry in patients and compared with the healthy control group .Results Compare with healthy control group ,the expressions of CD4+CD45RO+and CD8+CD45RO+ T lymphocyte in patients active stage was significantly increased (P<0 .01) ,but the expression of CD4+CD45RA+ and CD8+CD45RA+ decreased obviosly(P<0 .01) .The expres-sions of CD4+CD45RO+ and CD8+CD45RO+ T lymphocyte in patients stable stage was significantly lower than that in patients active stage(P<0 .05) ,but which was still higher than that in healthy control group (P<0 .05) .The ex-pression of CD4+CD45RA+ and CD8+CD45RA+ T lymphocyte in patients stable stage was significantly higher than that in patients active stage(P<0 .05) ,but which was still lower than that in healthy control group (P<0 .05) .Con-clusion The abnormal expresions of CD4+ CD45RO+ 、CD8+ CD45RO+ and CD4+ CD45RA+ 、CD8+ CD45RA+ T lymphocyte was one of the immportant reason for patients inmmune fuction disbalance with ankylosing spondylitis .
