检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2013年
16期
2100-2101
,共2页
舒芬太尼%酶联免疫吸附法%血药浓度%脐带血
舒芬太尼%酶聯免疫吸附法%血藥濃度%臍帶血
서분태니%매련면역흡부법%혈약농도%제대혈
sufentanil%enzyme-linked immunosorbent assay%plasma concentration%cord blood
目的探讨应用酶联免疫吸附法(ELISA)定量检测人血清舒芬太尼浓度及其在产妇镇痛分娩、婴儿脐带血血药浓度监测中的应用价值。方法应用ELISA两步法定量检测舒芬太尼浓度,拟合标准曲线,测定检测限,并检测自控椎管内镇痛分娩产妇和婴儿脐带血血清样本各48份,分别检测用药1、3、5 h和脐带血的血药浓度。结果此方法绘制的舒芬太尼标准曲线为:Y =420.375 X+32.043,1 h血清舒芬太尼浓度为(0.087±0.013)μg/m L ,3 h舒芬太尼浓度为(0.026±0.011)μg/m L ,5 h舒芬太尼浓度为(0.011±0.050)μg/m L ,脐带血血药浓度为(0.040±0.029)μg/mL。结论 ELISA方法测定人血舒芬太尼简便、快捷,血药浓度1 h达峰值,分娩镇痛效果理想,可用于临床药动学研究。婴儿脐带血药物浓度极低,未见药物不良反应。
目的探討應用酶聯免疫吸附法(ELISA)定量檢測人血清舒芬太尼濃度及其在產婦鎮痛分娩、嬰兒臍帶血血藥濃度鑑測中的應用價值。方法應用ELISA兩步法定量檢測舒芬太尼濃度,擬閤標準麯線,測定檢測限,併檢測自控椎管內鎮痛分娩產婦和嬰兒臍帶血血清樣本各48份,分彆檢測用藥1、3、5 h和臍帶血的血藥濃度。結果此方法繪製的舒芬太尼標準麯線為:Y =420.375 X+32.043,1 h血清舒芬太尼濃度為(0.087±0.013)μg/m L ,3 h舒芬太尼濃度為(0.026±0.011)μg/m L ,5 h舒芬太尼濃度為(0.011±0.050)μg/m L ,臍帶血血藥濃度為(0.040±0.029)μg/mL。結論 ELISA方法測定人血舒芬太尼簡便、快捷,血藥濃度1 h達峰值,分娩鎮痛效果理想,可用于臨床藥動學研究。嬰兒臍帶血藥物濃度極低,未見藥物不良反應。
목적탐토응용매련면역흡부법(ELISA)정량검측인혈청서분태니농도급기재산부진통분면、영인제대혈혈약농도감측중적응용개치。방법응용ELISA량보법정량검측서분태니농도,의합표준곡선,측정검측한,병검측자공추관내진통분면산부화영인제대혈혈청양본각48빈,분별검측용약1、3、5 h화제대혈적혈약농도。결과차방법회제적서분태니표준곡선위:Y =420.375 X+32.043,1 h혈청서분태니농도위(0.087±0.013)μg/m L ,3 h서분태니농도위(0.026±0.011)μg/m L ,5 h서분태니농도위(0.011±0.050)μg/m L ,제대혈혈약농도위(0.040±0.029)μg/mL。결론 ELISA방법측정인혈서분태니간편、쾌첩,혈약농도1 h체봉치,분면진통효과이상,가용우림상약동학연구。영인제대혈약물농도겁저,미견약물불량반응。
Objective To investigate value of enzyme-linked immunosorbent assay (ELISA ) quantitative de-tection serum sufentanil concentration ,analgesia delivery and the plasma concentrationcord in cord blood .Methods Used the two step methed of ELISA testing the plasma concentration of sufentanil ,fitting the standard curve to de-termine its detection limit ,and detect the plasma concentration of 48 samples which from the automatic control of in-traspinal analgesia and cord blood ,detect the plasma concentration when 1 hour ,3 hours ,5 hours andplasma concen-tration of cord blood .Results The method draws the sufentanil standard curve :Y = 420 .375X+ 32 .043 ,1 hours plasma concentration of sufentanil was (0 .087 ± 0 .013 )μg/mL ,3 hours plasma concentration of sufentanil was (0 .026 ± 0 .011)μg/mL ,5 hours plasma concentration of sufentanil was (0 .011 ± 0 .050)μg/mL ,The cord blood plasma concentration was (0 .040 ± 0 .029)μg/mL .Conclusion ELISA method for determination of serum sufentanil is simple ,fast .Ideal for labor analgesia ,it could be suitable for clinical pharmacokinetic study .umbilical cord blood-drug concentration in very small amounts and no adverse drug reaction .