中外医学研究
中外醫學研究
중외의학연구
CHINESE AND FOREIGN MEDICAL RESEARCH
2013年
21期
147-148
,共2页
磁粒子捕获免疫发光%酶联免疫吸附分析%癌胚抗原
磁粒子捕穫免疫髮光%酶聯免疫吸附分析%癌胚抗原
자입자포획면역발광%매련면역흡부분석%암배항원
Magnetic particle capture immune light%Enzyme linked immunosorbent analysis%Carcinoembryonic antigen
目的：比较磁粒子捕获免疫发光法(ICA)和酶联免疫吸附分析法(ELISA)测定血癌胚抗原(CEA)的结果，了解这两种方法的优劣，探讨ICA和ELISA检测CEA的灵敏性和准确性。方法：ICA和ELISA作对比试验、回收试验和精密度试验。结果：对比试验，ICA法所测CEA含量为Y值， ELISA所测CEA含量为X值，则回归方程：Y=1.32X+8.23，相关系数r=0.991；回收试验，用ICA和ELSIA平均回收率分别为97.64%和96.73%；精密度试验，ELISA和ICA的批内精密度分别为11.74%和1.92%，批间精密度分别15.41%和3.68%。结论：ICA与ELISA线性相关性较好，批内、批间精密度均优于ELISA法，ICA的检测精度高，稳定性强，回收率高于ELISA，ICA法在检测准确度和稳定性上，较之ELISA具有很强的优越性。
目的：比較磁粒子捕穫免疫髮光法(ICA)和酶聯免疫吸附分析法(ELISA)測定血癌胚抗原(CEA)的結果，瞭解這兩種方法的優劣，探討ICA和ELISA檢測CEA的靈敏性和準確性。方法：ICA和ELISA作對比試驗、迴收試驗和精密度試驗。結果：對比試驗，ICA法所測CEA含量為Y值， ELISA所測CEA含量為X值，則迴歸方程：Y=1.32X+8.23，相關繫數r=0.991；迴收試驗，用ICA和ELSIA平均迴收率分彆為97.64%和96.73%；精密度試驗，ELISA和ICA的批內精密度分彆為11.74%和1.92%，批間精密度分彆15.41%和3.68%。結論：ICA與ELISA線性相關性較好，批內、批間精密度均優于ELISA法，ICA的檢測精度高，穩定性彊，迴收率高于ELISA，ICA法在檢測準確度和穩定性上，較之ELISA具有很彊的優越性。
목적：비교자입자포획면역발광법(ICA)화매련면역흡부분석법(ELISA)측정혈암배항원(CEA)적결과，료해저량충방법적우렬，탐토ICA화ELISA검측CEA적령민성화준학성。방법：ICA화ELISA작대비시험、회수시험화정밀도시험。결과：대비시험，ICA법소측CEA함량위Y치， ELISA소측CEA함량위X치，칙회귀방정：Y=1.32X+8.23，상관계수r=0.991；회수시험，용ICA화ELSIA평균회수솔분별위97.64%화96.73%；정밀도시험，ELISA화ICA적비내정밀도분별위11.74%화1.92%，비간정밀도분별15.41%화3.68%。결론：ICA여ELISA선성상관성교호，비내、비간정밀도균우우ELISA법，ICA적검측정도고，은정성강，회수솔고우ELISA，ICA법재검측준학도화은정성상，교지ELISA구유흔강적우월성。
Objective:To compare magnetic particle capture immune luminescence(ICA) method and enzyme linked immunosorbent assay(ELISA) in determination of serum carcinoembryonic antigen (CEA) results,and understanding these two methods advantages and disadvantages and discuss ICA and ELISA detection of CEA sensitivity and accuracy.Methods:ICA and ELISA contrast test,recovery test and precision test.Results:Contrast test,Y was ICA method measured CEA content,X was ELISA measured CEA content,the regression equation:Y=1.32X+8.23,correlation coefficient r=0.991.Recovery test,with the ICA and ELSIA average recovery rate was 97.64%and 96.73%.The accuracy test,ELISA and the group of ICA in precision were 11.74%and 1.92%respectively. Batch precision was between 15.41%and 3.68%respectively.Conclusion:ICA and ELISA linear correlation is better,in the group,and group were superior to that of ELISA method between precision,ICA has high accuracy,stability is strong,ICA recovery than ELISA is high,the ICA in detection accuracy and stability compared with ELISA has a strong advantage.
