中国保健营养(中旬刊)
中國保健營養(中旬刊)
중국보건영양(중순간)
China Hwalth Care & nutrition
2013年
11期
43-44
,共2页
吴奎海%马均宝%崔东岚
吳奎海%馬均寶%崔東嵐
오규해%마균보%최동람
丝状真菌%内部转录间隔区%测序
絲狀真菌%內部轉錄間隔區%測序
사상진균%내부전록간격구%측서
filamentous fungi%internal transcribed spacer%ITS%sequencing
目的:建立以ITS2为靶序列鉴定临床分离丝状真菌的分子生物诊断方法。方法:利用通用引物扩增真菌核糖体转录间隔区2(ITS2),对PCR产物进行测序,在GenBank中对测序结果进行Blastn等分析。结果:9株真菌测序结果与表型鉴定符合,鉴定出7株临床分离丝状真菌。结论:ITS2测序可以作为临床丝状真菌的分子生物学诊断方法之一。
目的:建立以ITS2為靶序列鑒定臨床分離絲狀真菌的分子生物診斷方法。方法:利用通用引物擴增真菌覈糖體轉錄間隔區2(ITS2),對PCR產物進行測序,在GenBank中對測序結果進行Blastn等分析。結果:9株真菌測序結果與錶型鑒定符閤,鑒定齣7株臨床分離絲狀真菌。結論:ITS2測序可以作為臨床絲狀真菌的分子生物學診斷方法之一。
목적:건립이ITS2위파서렬감정림상분리사상진균적분자생물진단방법。방법:이용통용인물확증진균핵당체전록간격구2(ITS2),대PCR산물진행측서,재GenBank중대측서결과진행Blastn등분석。결과:9주진균측서결과여표형감정부합,감정출7주림상분리사상진균。결론:ITS2측서가이작위림상사상진균적분자생물학진단방법지일。
Objective:To establish a method of sequencing of internal transcribed spacer 2 (ITS2) for identification of filamentous fungi from clinical specimens. Methods:Amplification and sequencing of internal transcribed spacer 2 (ITS2) region of fungi were performed using universal primers. Searches of current nucleotide databases in GenBank were carried out with the BLAST algorithm. Results:9 clinical common fungal isolates were accurately identified . 6 clinical filamentous fungi isolates were detected. Conclusions:ITS2 sequencing for ilamentous fungi is an excellent method.