CAJ | 학술논문

　　目的研究低频超声能否增强双氢青蒿素（DHA）对前列腺癌PC-3细胞种植瘤的致凋亡作用，并探讨其对DHA诱导前列腺癌PC-3细胞凋亡增敏作用的机理。方法采用前列腺癌 PC-3细胞建立裸鼠种植瘤模型，20只实验裸鼠随机分为4组：空白对照组、单纯DHA组、单纯超声组和超声＋DHA组，经过13 d共计7次药物或加超声干预后，采用免疫组织化学法（SP）检测凋亡相关基因Bcl-2、Bax、VEGF、Caspase-3及CHOP的表达水平。结果观察瘤体外形见空白对照组与单纯超声组肿瘤体积稍大，切开瘤体见内部均有明显液化坏死区域，免疫组织化学结果显示超声＋DHA组、单纯DHA组和单纯超声组的Bcl-2及VEGF表达均低于空白对照组，超声＋DHA组、DHA组和超声组的Bax、Caspase-3及CHOP表达均高于空白组。结论对正常细胞不致明显损伤的低频超声，可致前列腺癌PC-3种植瘤细胞相关凋亡基因的改变，D H A可单独致PC-3细胞凋亡及相关基因的改变，超声能明显增强D H A诱导前列腺癌PC-3种植瘤细胞凋亡作用。
　　목적연구저빈초성능부증강쌍경청호소（DHA）대전렬선암PC-3세포충식류적치조망작용，병탐토기대DHA유도전렬선암PC-3세포조망증민작용적궤리。방법채용전렬선암 PC-3세포건립라서충식류모형，20지실험라서수궤분위4조：공백대조조、단순DHA조、단순초성조화초성＋DHA조，경과13 d공계7차약물혹가초성간예후，채용면역조직화학법（SP）검측조망상관기인Bcl-2、Bax、VEGF、Caspase-3급CHOP적표체수평。결과관찰류체외형견공백대조조여단순초성조종류체적초대，절개류체견내부균유명현액화배사구역，면역조직화학결과현시초성＋DHA조、단순DHA조화단순초성조적Bcl-2급VEGF표체균저우공백대조조，초성＋DHA조、DHA조화초성조적Bax、Caspase-3급CHOP표체균고우공백조。결론대정상세포불치명현손상적저빈초성，가치전렬선암PC-3충식류세포상관조망기인적개변，D H A가단독치PC-3세포조망급상관기인적개변，초성능명현증강D H A유도전렬선암PC-3충식류세포조망작용。
Objective To study the enhancement effects of the low frequency ultrasound to the apoptosis induced by dihydroar-temisinin in the transplantation human prostate cancer PC-3 cells in nude mice ,and to explore the underlying action mechanism . Methods Prostate cancer PC-3 cells were transplanted into 20 nude mice to establish the solid tumor mode .These nude mice were randomly divided into 4 groups with 5 mice in each group :control group ,dihydroartemisinin group ,ultrasound group and ultrasound+ dihydroartemisinin group .After 13 days and 7 times drug administration ,The positive products of Bcl-2 ,Bax ,VEGF ,Caspase-3 and CHOP were tested by immunohistochemical method .Results The tumor volume were larger in control group and ultrasound group through observed by tumor contour ,and there were liquefication and necrosis region after incised the tumor tissue .Immuno-histochemical examination revealed that the positive products of Bcl-2 and VEGF were decreased in ultrasound+DHA group ,DHA group and ultrasound group .Immunohistochemical examination revealed that the positive products of Bax 、Caspase-3 and CHOP were significantly increased in ultrasound+DHA group ,DHA group and ultrasound group .Conclusion The low frequency ultra-sound can′t cause obvious damage to the normal cell ,which could causing the change of the positive products in transplantation hu-man prostate cancer PC-3 cells .The results show that DHA has this action alone ,the ultrasound may enhance the apoptosis of PC-3 cells induced by DHA.