CAJ | 학술논문

　　目的构建人疱疹病毒8型（HHV-8）融合蛋白原核表达载体，为诊断 HHV-8感染奠定基础。方法构建 HHV-8融合蛋白原核表达载体转化大肠杆菌DH5α，诱导表达融合蛋白ORF59-ORF65-K8．1。聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹（Western blot）法鉴定融合蛋白。融合蛋白对无偿献血人员血清进行检测。结果 SDS-PAGE显示融合蛋白约24 KD ，与预期值一致；Western blot提示该融合蛋白可以和 HHV-8阳性血清特异性结合。融合蛋白包被酶连免疫吸附实验（ELISA）板后与无偿献血人员血清反应检测 HHV-8感染情况，检测结果与市售 HHV-8 ELISA试剂盒检测结果一致。结论本文构建的融合蛋白可作为 HHV-8感染的检测抗原用来筛查献血人员 HHV-8感染情况及普通人群 HHV-8感染流行病学调查。
　　목적구건인포진병독8형（HHV-8）융합단백원핵표체재체，위진단 HHV-8감염전정기출。방법구건 HHV-8융합단백원핵표체재체전화대장간균DH5α，유도표체융합단백ORF59-ORF65-K8．1。취병희선알응효전영（SDS-PAGE）화면역인적（Western blot）법감정융합단백。융합단백대무상헌혈인원혈청진행검측。결과 SDS-PAGE현시융합단백약24 KD ，여예기치일치；Western blot제시해융합단백가이화 HHV-8양성혈청특이성결합。융합단백포피매련면역흡부실험（ELISA）판후여무상헌혈인원혈청반응검측 HHV-8감염정황，검측결과여시수 HHV-8 ELISA시제합검측결과일치。결론본문구건적융합단백가작위 HHV-8감염적검측항원용래사사헌혈인원 HHV-8감염정황급보통인군 HHV-8감염류행병학조사。
Objective To construct the prokaryotic expression plasmid of HHV-8 fusion antigen for diagnosis of HHV-8 infec-tion .Methods The combined fragment ORF59 ,ORF65 and K8 .1 by fusion PCR was integrated into pQE-80L and transfected into E .coli DH5α.Fusion protein was induced to express by IPTG .SDS-PAGE and Western blot were employed to detect the fusion protein .Fusion protein was used to detect serum of blood donors .Results The combined plasmid pQE-80L-ORF59-ORF65-K8 .1 was constructed successfully after verifying by restriction enzyme digestion and sequencing .The fusion protein was about 24 KD and could be specific combined with HHV-8 positive serum .The fusion protein had the same result to detect HHV-8 with the HHV-8 ELISA kit .Conclusion Fusion protein we construct can be used as diagnosis antigen to detect HHV-8 of blood donors and common people .