当代医学
噹代醫學
당대의학
CHINA CONTEMPORARY MEDICINE
2013年
24期
1-2
,共2页
吴恺%谭钢%邵毅%刘二华
吳愷%譚鋼%邵毅%劉二華
오개%담강%소의%류이화
莱菔硫烷%血红素氧合酶1%核因子相关因子2%晶状体上皮细胞
萊菔硫烷%血紅素氧閤酶1%覈因子相關因子2%晶狀體上皮細胞
래복류완%혈홍소양합매1%핵인자상관인자2%정상체상피세포
Sulforaphane%Heme oxygenase 1%Nuclear factor erythroid 2-related factor 2%Lens epithelial cells
目的研究莱菔硫烷对高糖刺激下大鼠晶状体上皮细胞血红素氧合酶1(hemeoxygenase-1,HO-1)和核因子相关因子2(nuclear factor erythroid 2-related factor 2, Nrf 2)表达的影响。方法人LECs系SRA 01/04细胞用含葡萄糖5.5 mmol/L(正常对照组)和30.5 mmol/L(高糖组)的培养液中培养。观察组用不同浓度SFN处理24 h后,Western blot观察HO-1的表达以及Nrf 2的核转位。结果正常对照组SRA 01/04细胞中HO-1表达量非常低,高糖处理后,HO-1表达水平有所增加。但SFN处理后,HO-1的表达随着SFN浓度的增高而进一步增高;SRA 01/04细胞在正常对照组中Nrf 2主要位于细胞浆内,细胞核中Nrf 2含量极低。高糖组细胞核中Nrf 2仅轻度增加。而SFN处理后,细胞核内Nrf 2表达量显著增高。此外,SFN组经Nrf 2 siRNA干扰后,HO-1的表达量显著降低。结论 SFN可通过激活Nrf 2诱导大鼠晶状体上皮细胞表达HO-1,从而发挥抗氧化作用。
目的研究萊菔硫烷對高糖刺激下大鼠晶狀體上皮細胞血紅素氧閤酶1(hemeoxygenase-1,HO-1)和覈因子相關因子2(nuclear factor erythroid 2-related factor 2, Nrf 2)錶達的影響。方法人LECs繫SRA 01/04細胞用含葡萄糖5.5 mmol/L(正常對照組)和30.5 mmol/L(高糖組)的培養液中培養。觀察組用不同濃度SFN處理24 h後,Western blot觀察HO-1的錶達以及Nrf 2的覈轉位。結果正常對照組SRA 01/04細胞中HO-1錶達量非常低,高糖處理後,HO-1錶達水平有所增加。但SFN處理後,HO-1的錶達隨著SFN濃度的增高而進一步增高;SRA 01/04細胞在正常對照組中Nrf 2主要位于細胞漿內,細胞覈中Nrf 2含量極低。高糖組細胞覈中Nrf 2僅輕度增加。而SFN處理後,細胞覈內Nrf 2錶達量顯著增高。此外,SFN組經Nrf 2 siRNA榦擾後,HO-1的錶達量顯著降低。結論 SFN可通過激活Nrf 2誘導大鼠晶狀體上皮細胞錶達HO-1,從而髮揮抗氧化作用。
목적연구래복류완대고당자격하대서정상체상피세포혈홍소양합매1(hemeoxygenase-1,HO-1)화핵인자상관인자2(nuclear factor erythroid 2-related factor 2, Nrf 2)표체적영향。방법인LECs계SRA 01/04세포용함포도당5.5 mmol/L(정상대조조)화30.5 mmol/L(고당조)적배양액중배양。관찰조용불동농도SFN처리24 h후,Western blot관찰HO-1적표체이급Nrf 2적핵전위。결과정상대조조SRA 01/04세포중HO-1표체량비상저,고당처리후,HO-1표체수평유소증가。단SFN처리후,HO-1적표체수착SFN농도적증고이진일보증고;SRA 01/04세포재정상대조조중Nrf 2주요위우세포장내,세포핵중Nrf 2함량겁저。고당조세포핵중Nrf 2부경도증가。이SFN처리후,세포핵내Nrf 2표체량현저증고。차외,SFN조경Nrf 2 siRNA간우후,HO-1적표체량현저강저。결론 SFN가통과격활Nrf 2유도대서정상체상피세포표체HO-1,종이발휘항양화작용。
Objective To elucidate the antioxidant effect of Sulforaphane (SFN) in on the expression of hemeoxygenase 1 (HO-1) and nuclear factor erythroid 2-related factor 2 ( Nrf 2) in rat lens epithelial cells cultured in vitro with high glucose.Methods Human lens epithelial cell line SRA 01/04 was cultured in vitro containing 5.5 mmol/L or 30.5 mmol/L glucose as control and high-glucose group, repectively. The observation group was treated by different concentration of SFN for 24 h, expression of heme oxygenase 1 (HO-1) and nuclear translocation were detected by Western blot. Results Expression of HO-1 in the control group was very low, and high-glucose could slightly increase the HO-1 level. SFN treatment could increase the expression of HO-1 in dose-dependent manner. Nrf 2 was in the cytoplasm, and was very low in nucleolus. high-glucose could only increase Nrf 2 nuclear translocation by mild. However, SFN could signiifcantly promote Nrf 2 translocate to the nucleus, In addition, silencing of Nrf 2 could profoundly inhibit SFN-induced HO-1 expression. Conclusion SFN could induce rat lens epithelial cells expression of HO-1, and this mechanism may be involved in beneifcial effect against high glucose-induced oxidative stress.