CAJ | 학술논문

目的：体外培养肝星状细胞（HSC），从分子生物学的角度探索其是否有ACE 2和Mas受体mRNA的表达，以及血管紧张素转换酶抑制剂(ACEI)贝那普利对二者的影响。方法大鼠肝星状细胞株(HSC-T 6)按实验计划分组处理（正常对照组、AngⅡ组、AngⅡ＋贝那普利组、贝那普利组），提取总RNA，逆转录后应用RTFQ PCR的方法测量ACE 2及Mas受体 mRNA的基因水平。结果 ACE 2、Mas受体mRNA在各组中均有表达；与对照组相比，AngⅡ组中二者的表达均较高，差异有统计学意义（P<0.05）；在AngⅡ＋贝那普利组，二者均较AngⅡ组升高，差异有统计学意义（P<0.05）。结论 AngⅡ可使肝星状细胞激活，活化后的HSC的ACE 2、Mas受体mRNA的基因表达水平升高，从而发挥其抗肝纤维化的作用。
목적：체외배양간성상세포（HSC），종분자생물학적각도탐색기시부유ACE 2화Mas수체mRNA적표체，이급혈관긴장소전환매억제제(ACEI)패나보리대이자적영향。방법대서간성상세포주(HSC-T 6)안실험계화분조처리（정상대조조、AngⅡ조、AngⅡ＋패나보리조、패나보리조），제취총RNA，역전록후응용RTFQ PCR적방법측량ACE 2급Mas수체 mRNA적기인수평。결과 ACE 2、Mas수체mRNA재각조중균유표체；여대조조상비，AngⅡ조중이자적표체균교고，차이유통계학의의（P<0.05）；재AngⅡ＋패나보리조，이자균교AngⅡ조승고，차이유통계학의의（P<0.05）。결론 AngⅡ가사간성상세포격활，활화후적HSC적ACE 2、Mas수체mRNA적기인표체수평승고，종이발휘기항간섬유화적작용。
Objective To investigate the efects of angiotensin converting enzyme inhibitors benazepril on angiotensin converting enzyme 2(ACE 2) and Mas receptor production of in vitro cultured hepatic stellate cells (HSC). Methods HSC-T 6 rat hepatic stelate was chosen as the main study model of experiment,Cultured HSC were randomized into control group,AngⅡgroup,benazepril group and AngⅡ+benazepril group. The expression of ACE 2 and Mas receptor in different group was measured by by RTFQ PCR.Results The ACE 2 and Mas receptor expression level of HSC in AngⅡgroup were higher than control group(P<0.05).All index of AngⅡ+benazepril group were higher than AngⅡgroup (P<0.05). Conclusion After hepatic stelate cells was activated,Benazepril can elevated the ACE 2 and Mas receptor expression level of HSC,and play an important role in anti-hepatic fibrosis.