中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
3期
47-49
,共3页
原发性肝癌%甲胎蛋白异质体%甲胎蛋白信使核糖核酸%诊断%预后
原髮性肝癌%甲胎蛋白異質體%甲胎蛋白信使覈糖覈痠%診斷%預後
원발성간암%갑태단백이질체%갑태단백신사핵당핵산%진단%예후
Primary liver cancer%Alpha fetoprotein heterogeneity%Alpha fetoprotein messenger ribonucleic acid%Diagnosis%Prognosis
目的：探讨联合检测2种血清肿瘤标志物甲胎蛋白（AFP）异质体（AFP-L3）和甲胎蛋白信使核糖核酸（AFPmRNA）诊断肝细胞癌价值及评估肝癌预后的意义。方法：对2005年4-7月收治的82例肝细胞癌（HCC）、11例慢性乙型肝炎肝硬化合并门脉高压和19例肝脏良性肿瘤患者应用酶联吸附法及荧光定量PCR联合检测AFP-L3和AFPmRNA水平，对比其阳性率；并对术后1个月的肝癌患者检测AFP-L3和AFPmRNA水平，统计作出生存曲线。结果：术前不同肿瘤指标的检测结果：AFP-L3联合AFPmRNA检测阳性率为64.3%，明显高于AFP、AFP-mRNA单独检测的阳性率（50.9%、40.2%），差异均有统计学意义（P<0.05）；术前、术后AFP-L3联合AFPmRNA检测均阳性的患者术后3年生存率较低。结论：术前联合检测APF-L3和AFPmRNA两项指标可提高HCC、尤其是AFP阴性HCC的诊断率，而术后联合检测又对评估HCC的预后有一定的指导意义。
目的：探討聯閤檢測2種血清腫瘤標誌物甲胎蛋白（AFP）異質體（AFP-L3）和甲胎蛋白信使覈糖覈痠（AFPmRNA）診斷肝細胞癌價值及評估肝癌預後的意義。方法：對2005年4-7月收治的82例肝細胞癌（HCC）、11例慢性乙型肝炎肝硬化閤併門脈高壓和19例肝髒良性腫瘤患者應用酶聯吸附法及熒光定量PCR聯閤檢測AFP-L3和AFPmRNA水平，對比其暘性率；併對術後1箇月的肝癌患者檢測AFP-L3和AFPmRNA水平，統計作齣生存麯線。結果：術前不同腫瘤指標的檢測結果：AFP-L3聯閤AFPmRNA檢測暘性率為64.3%，明顯高于AFP、AFP-mRNA單獨檢測的暘性率（50.9%、40.2%），差異均有統計學意義（P<0.05）；術前、術後AFP-L3聯閤AFPmRNA檢測均暘性的患者術後3年生存率較低。結論：術前聯閤檢測APF-L3和AFPmRNA兩項指標可提高HCC、尤其是AFP陰性HCC的診斷率，而術後聯閤檢測又對評估HCC的預後有一定的指導意義。
목적：탐토연합검측2충혈청종류표지물갑태단백（AFP）이질체（AFP-L3）화갑태단백신사핵당핵산（AFPmRNA）진단간세포암개치급평고간암예후적의의。방법：대2005년4-7월수치적82례간세포암（HCC）、11례만성을형간염간경화합병문맥고압화19례간장량성종류환자응용매련흡부법급형광정량PCR연합검측AFP-L3화AFPmRNA수평，대비기양성솔；병대술후1개월적간암환자검측AFP-L3화AFPmRNA수평，통계작출생존곡선。결과：술전불동종류지표적검측결과：AFP-L3연합AFPmRNA검측양성솔위64.3%，명현고우AFP、AFP-mRNA단독검측적양성솔（50.9%、40.2%），차이균유통계학의의（P<0.05）；술전、술후AFP-L3연합AFPmRNA검측균양성적환자술후3년생존솔교저。결론：술전연합검측APF-L3화AFPmRNA량항지표가제고HCC、우기시AFP음성HCC적진단솔，이술후연합검측우대평고HCC적예후유일정적지도의의。
Objective:To investigate the combined detection of 2 tumor markers in serum alpha fetoprotein(AFP)variants(AFP-L3)and alpha fetoprotein messenger ribonucleic acid(AFPmRNA)value and evaluate prognosis significance in diagnosis of hepatocellular carcinoma.Method:From April to July 2005,82 cases of hepatocellular carcinoma(HCC),11 patients with chronic hepatitis B cirrhosis with portal hypertension and 19 cases of benign liver tumors were treated with enzyme-linked assay and fluorescence quantitative PCR and detection of AFP-L3 and AFPmRNA level,compared the positive rate;and 1 month after the detection of liver cancer patients and AFPmRNA AFP-L3 level statistics make survival curve. Result:Through AFPmRNA and AFP-L3 joint detection,positive rate was 64.3%,significantly higher than the separate detecting AFP-L3,AFP,AFP-mRNA positive rate was high (50.9%,40.2%). And there were statistically significant(P<0.05);Preoperative and postoperative joint detection of AFP-L3 and AFPmRNA positive patients with postoperative 3 year survival rate were low. Conclusion:Preoperative two indexes of combined detection of APF-L3 and AFPmRNA can improve the diagnosis of HCC,especially AFP negative rate of HCC,and after the combined detection of HCC and to evaluate the clinical significance.
