CAJ | 학술논문

目的：探讨2型糖尿病（DM）醛糖还原酶（AR）基因5′端的（AC）n的多态性与糖尿病肾病（DN）、糖尿病周围神经病变（DPN）易感性的关系。方法：选择本院2型DM患者119例为DM组，根据其同时伴（+）或不伴（-）DN与DPN，将DM组分为四个亚组：DN-/DPN-组、DN-/DPN+组、DN+/DPN-组、DN+/DPN+组；同时选取健康体检者62例为正常对照（CON）组。采用聚合酶链反应（PCR）及尿素/甲酰胺聚丙烯酰胺凝胶电泳银染显色法分析各组AR基因的二核苷酸（AC）n串联重复序列的多态标记，比较各组间等位基因频率和基因型频率。结果：各组中Z-2等位基因频率比较差异有统计学意义（P<0.05），其中在DN+/DPN+组最高，在DN-/DPN-组最低；Z+2等位基因频率在DN-/DPN-组显著增高，在DN+/DPN+组显著降低，两组比较差异有统计学意义（P<0.01）；纯合子Z-2/Z-2基因型频率在DN+/DPN+组显著高于其余各组，而纯合子Z+2/Z+2基因型频率在DN-/DPN-组显著高于其余各组。结论：Z+2等位基因是2型糖尿病患者发生DN及DPN的保护性基因；Z-2等位基因是DN易感基因，而病程和Z-2等位基因可能共同促进了DPN的发生。
목적：탐토2형당뇨병（DM）철당환원매（AR）기인5′단적（AC）n적다태성여당뇨병신병（DN）、당뇨병주위신경병변（DPN）역감성적관계。방법：선택본원2형DM환자119례위DM조，근거기동시반（+）혹불반（-）DN여DPN，장DM조분위사개아조：DN-/DPN-조、DN-/DPN+조、DN+/DPN-조、DN+/DPN+조；동시선취건강체검자62례위정상대조（CON）조。채용취합매련반응（PCR）급뇨소/갑선알취병희선알응효전영은염현색법분석각조AR기인적이핵감산（AC）n천련중복서렬적다태표기，비교각조간등위기인빈솔화기인형빈솔。결과：각조중Z-2등위기인빈솔비교차이유통계학의의（P<0.05），기중재DN+/DPN+조최고，재DN-/DPN-조최저；Z+2등위기인빈솔재DN-/DPN-조현저증고，재DN+/DPN+조현저강저，량조비교차이유통계학의의（P<0.01）；순합자Z-2/Z-2기인형빈솔재DN+/DPN+조현저고우기여각조，이순합자Z+2/Z+2기인형빈솔재DN-/DPN-조현저고우기여각조。결론：Z+2등위기인시2형당뇨병환자발생DN급DPN적보호성기인；Z-2등위기인시DN역감기인，이병정화Z-2등위기인가능공동촉진료DPN적발생。
Objective:To study the relationship between the polymorphism of(AC)n in 5′end of the AR gene and the susceptibility of diabetic nephropathy,diabetic peripheral neuropathy in type 2 DM. Method:Chosen our hospital 119 patients with type 2 DM for DM group,at the same time, according to its companion(+)or without(-)DN and DPN,DM group were divided into four subgroups:DN/DPN-group,the DN/DPN+group,DN+/DPN-group,the DN+/DPN+group;At the same time chosen 62 cases of healthy check-up as normal control group(CON). The polymerase chain reaction(PCR)by urea,formamide and polyacrylamide gel electrophoresis,silver dye chromogenic method AR gene dinucleotide(AC)n polymorphism of tandem repeat sequence tags were used and analyze in each group,compared the allele frequency and genotype frequency of each group. Result:The frequence of Z-2 allele in DN+/DPN+group was much higher than that in the other groups(P<0.05). The frequence of Z+2 allele in group DN+/DPN+was much lower than that in the other groups(P<0.01);Z-2/Z-2 genotype frequency in the DN+/DPN+group was significantly higher than that of the other groups,and Z+2/Z+2 genotype frequency in the DN-/DPN-group was significantly higher than the other groups. Conclusion:AR gene alleles are associated with the development of diabetic microangiopathy. The Z+2 allele protect against the development and progression of DN and DPN. Whereas, the Z-2 allele may be a risk factor for the developing of DN. Both the course of DM and Z-2 allele promote the development of DNP.