CAJ | 학술논문

目的：应用荧光原位杂交技术(fluorescence in situ hybridization,FISH)对膀胱尿路上皮肿瘤患者术后复发进行早期评估预测。方法2009年1月至2011年11月,选取首都医科大学附属北京同仁医院40例临床诊断的高危膀胱尿路上皮癌患者,术后第3,6,9,12,18个月行尿细胞学检查、膀胱镜检查,并行尿液 FISH 检查,记录膀胱肿瘤复发的时间。观察 FISH 对于膀胱尿路上皮肿瘤患者术后复发的早期预测价值。结果60%(12/20)的 FISH 阳性者膀胱肿瘤复发,FISH 阴性者无1例出现膀胱肿瘤复发。 FISH 阳性者膀胱尿路上皮癌复发率高于 FISH 阴性者(60% vs 0%,P<0.05)。6例(50%)肿瘤复发患者 FISH 阳性先于膀胱镜检查及尿细胞学检查。结论 FISH 对膀胱肿瘤检测的阳性率高于细胞学检测,FISH 对膀胱肿瘤的诊断早于膀胱镜和细胞学。 FISH 技术可作为膀胱尿路上皮肿瘤术后复发的监测指标之一。
목적：응용형광원위잡교기술(fluorescence in situ hybridization,FISH)대방광뇨로상피종류환자술후복발진행조기평고예측。방법2009년1월지2011년11월,선취수도의과대학부속북경동인의원40례림상진단적고위방광뇨로상피암환자,술후제3,6,9,12,18개월행뇨세포학검사、방광경검사,병행뇨액 FISH 검사,기록방광종류복발적시간。관찰 FISH 대우방광뇨로상피종류환자술후복발적조기예측개치。결과60%(12/20)적 FISH 양성자방광종류복발,FISH 음성자무1례출현방광종류복발。 FISH 양성자방광뇨로상피암복발솔고우 FISH 음성자(60% vs 0%,P<0.05)。6례(50%)종류복발환자 FISH 양성선우방광경검사급뇨세포학검사。결론 FISH 대방광종류검측적양성솔고우세포학검측,FISH 대방광종류적진단조우방광경화세포학。 FISH 기술가작위방광뇨로상피종류술후복발적감측지표지일。
Objective To early diagnose postoperative recurrence of bladder urothelial cancer with fluorescence in situ hybridization (FISH). Methods Forty patients with bladder urothelial cancer were chosen between January, 2009 and November, 2011. Urine cytologic examination, cystoscopy, and FISH were performed in these patients at 3, 6, 9, 12, and 18 months post-operation, and tumor recurrence was recorded. Results Among the 40 patients tested, 12 of 20 FISH-positive patients developed tumor recurrence within the follow-up period, while none of the FISH-negative patients developed recurrence during the same period. The recurrence rate in FISH-positive patients(60% ) was significantly higher than that of FISH-negative patients(P<0. 05). The sensitivity to detect tumor recurrence by FISH analysis was higher than that by cystoscopy and urine cytologic examination. Conclusion FISH analysis is effective in early detection of postoperative recurrence of bladder urothelial cancer.