浙江农业学报
浙江農業學報
절강농업학보
ACTA AGRICULTURAE ZHEJIANGENSIS
2014年
2期
335-338
,共4页
紫花亚菊%茎叶外植体%再生体系
紫花亞菊%莖葉外植體%再生體繫
자화아국%경협외식체%재생체계
Ajania purpurea%explants from stems and leaves%regeneration system
以紫花亚菊（Ajania purpurea）组培苗茎段、叶片为外植体，在附加不同浓度植物生长调节剂6-BA和NAA的MS培养基上诱导培养，获得再生植株。对试验结果进行观察分析筛选出合适的配方：愈伤组织诱导培养基为MS＋1.0 mg?L-16-BA＋0.1 mg?L-1 NAA，诱导率达95.6％；芽诱导培养基为： MS＋1.0 mg?L-16-BA＋0.2 mg?L-1 NAA，诱导率达82％；不定根最适诱导培养基为：1/2 MS＋0.15 mg?L-1 IBA，生根率达90％以上，再生植株移栽成活率达90％。
以紫花亞菊（Ajania purpurea）組培苗莖段、葉片為外植體，在附加不同濃度植物生長調節劑6-BA和NAA的MS培養基上誘導培養，穫得再生植株。對試驗結果進行觀察分析篩選齣閤適的配方：愈傷組織誘導培養基為MS＋1.0 mg?L-16-BA＋0.1 mg?L-1 NAA，誘導率達95.6％；芽誘導培養基為： MS＋1.0 mg?L-16-BA＋0.2 mg?L-1 NAA，誘導率達82％；不定根最適誘導培養基為：1/2 MS＋0.15 mg?L-1 IBA，生根率達90％以上，再生植株移栽成活率達90％。
이자화아국（Ajania purpurea）조배묘경단、협편위외식체，재부가불동농도식물생장조절제6-BA화NAA적MS배양기상유도배양，획득재생식주。대시험결과진행관찰분석사선출합괄적배방：유상조직유도배양기위MS＋1.0 mg?L-16-BA＋0.1 mg?L-1 NAA，유도솔체95.6％；아유도배양기위： MS＋1.0 mg?L-16-BA＋0.2 mg?L-1 NAA，유도솔체82％；불정근최괄유도배양기위：1/2 MS＋0.15 mg?L-1 IBA，생근솔체90％이상，재생식주이재성활솔체90％。
A plant regeneration system from leaves and stems of tissue-cultured Ajania purpurea was developed in this study.The results showed that the callus was induced with a rate of 95.6%on an optimal medium MS+1.0 mg?L-1 6-BA+0.1 mg?L-1 NAA.The highest induction rate of the adventitious shoots from the calli on the medium of MS+1.0 mg?L-1 6-BA+0.2 mg?L-1 NAA was 82%.The optimal medium for adventitious root inducing was 1/2MS+0.15 mg?L-1 IBA, with a rooting rate of 90%.The plantlets were transplanted to pots with sands , vermiculite and peat to acclimate for 2 -3 weeks.Then the survival plantlets were transferred outdoors with 90% transplanting survival rate.
