CAJ | 학술논문

目的：探索睾丸支持细胞（Sertoli细胞）对骨髓间充质干细胞（BMSC）免疫抑制作用的影响，为二者在移植免疫中的联合应用提供思路。方法二步酶解法处理大鼠睾丸，分离Sertoli细胞；Percoll法分离大鼠BMSC；Ficoll法分离淋巴细胞；刀豆蛋白A（ConA）刺激进行T细胞转化试验；将Sertoli细胞、BMSC和Sertoli细胞＋BMSC分别加入未经ConA处理的静止淋巴细胞培养体系和经ConA处理后的T细胞转化体系，MTT法测定淋巴细胞增殖情况，观察BMSC、Sertoli细胞或二者共培养对T细胞活化、增殖的影响。结果 BMSC、Sertoli细胞以及二者共培养对静止的淋巴细胞无明显作用。 BMSC、Sertoli细胞及二者共培养对 T细胞的活化、增殖均有明显的抑制作用，且Sertoli细胞与BMSC共培养时抑制作用呈现一定的协同性。结论 BMSC和Sertoli细胞均具有负性免疫调节作用，二者共培养可以进一步增强BMSC的免疫抑制效应。
목적：탐색고환지지세포（Sertoli세포）대골수간충질간세포（BMSC）면역억제작용적영향，위이자재이식면역중적연합응용제공사로。방법이보매해법처리대서고환，분리Sertoli세포；Percoll법분리대서BMSC；Ficoll법분리림파세포；도두단백A（ConA）자격진행T세포전화시험；장Sertoli세포、BMSC화Sertoli세포＋BMSC분별가입미경ConA처리적정지림파세포배양체계화경ConA처리후적T세포전화체계，MTT법측정림파세포증식정황，관찰BMSC、Sertoli세포혹이자공배양대T세포활화、증식적영향。결과 BMSC、Sertoli세포이급이자공배양대정지적림파세포무명현작용。 BMSC、Sertoli세포급이자공배양대 T세포적활화、증식균유명현적억제작용，차Sertoli세포여BMSC공배양시억제작용정현일정적협동성。결론 BMSC화Sertoli세포균구유부성면역조절작용，이자공배양가이진일보증강BMSC적면역억제효응。
Objective To investigate the effects of Sertoli cells on the immunosuppressive activity of bone marrow mesenchymal stem cells (BMSCs).Methods Sertoli cells were isolated using two steps enzyme digesting method from rat testicle .BMSCs were prepared using Percoll density gradient centrifugation method .Proliferation of lymphocytes induced by ConA was measured using MTT method.The effects of BMSCs and Sertoli cells on ConA-induced T lymphocyte transformation were investigated .Results BMSCs and Sertoli cells were shown to significantly inhibit T lymphocyte transformation induced by ConA .The inhibitory effects of BMSCs and Sertoli cells co-culture system were stronger than that of BMSCs alone .Conclusions BMSCs and Sertoli cells exert immunosuppressive effects.Sertoli cells can improve the effects of BMSCs .