延边大学学报(自然科学版)
延邊大學學報(自然科學版)
연변대학학보(자연과학판)
JOURNAL OF YANBIAN UNIVERSITY(NATURAL SCIENCE EDITION)
2013年
4期
269-272
,共4页
提取%测定%野生及种植灵芝%灵芝酸%总黄酮
提取%測定%野生及種植靈芝%靈芝痠%總黃酮
제취%측정%야생급충식령지%령지산%총황동
extraction%determination%wild and planted Ganoderma lucidum%ganoderic acid%total flavonoids
采用回流的方法从野生及种植灵芝中提取灵芝酸和总黄酮,用紫外分光光度法测定其含量.测得野生及种植灵芝中灵芝酸含量分别为0.366%和0.871%,相对标准偏差(RSD,n=4)分别为1.07%和0.81%,平均回收率为99.65%;野生及种植灵芝中总黄酮含量分别为0.507%和0.551%,RSD(n=4)分别为1.31%和1.10%,平均回收率为99.39%.
採用迴流的方法從野生及種植靈芝中提取靈芝痠和總黃酮,用紫外分光光度法測定其含量.測得野生及種植靈芝中靈芝痠含量分彆為0.366%和0.871%,相對標準偏差(RSD,n=4)分彆為1.07%和0.81%,平均迴收率為99.65%;野生及種植靈芝中總黃酮含量分彆為0.507%和0.551%,RSD(n=4)分彆為1.31%和1.10%,平均迴收率為99.39%.
채용회류적방법종야생급충식령지중제취령지산화총황동,용자외분광광도법측정기함량.측득야생급충식령지중령지산함량분별위0.366%화0.871%,상대표준편차(RSD,n=4)분별위1.07%화0.81%,평균회수솔위99.65%;야생급충식령지중총황동함량분별위0.507%화0.551%,RSD(n=4)분별위1.31%화1.10%,평균회수솔위99.39%.
Ganoderic acid and total flavonoids in the wild and planted Ganoderma lucidum are extracted by reflux method and determined by ultraviolet spectroscopy.Ganoderic acid in the wild and planted Ganoderma lucidum is 0.366% and 0.871%,respectively.Relative standard deviation (RSD)(%)(n=4)is 1.07% and 0.81%,the average recovery is 99.65%.Total flavonoids in the wild and planted Ganoderma lucidum is 0.507% and 0.551%,RSD(%)(n=4)is 1.31% and 1.10%,the average recovery is 99.39%.