CAJ | 학술논문

目的：观察EGCG对可卡因小鼠自发活动的影响，并探讨其作用机制。方法通过小鼠腹腔注射可卡因，预先给予EGCG进行干预，采用行为学检测小鼠的自发活动，采用Western blot 的方法检测小鼠伏隔核PICK1蛋白的表达。结果EGCG可抑制可卡因所致小鼠自发活动的增强，并且减少小鼠伏隔核 PICK1蛋白表达。结论EGCG可能对可卡因所致的药物成瘾有防治作用，其作用可能与抑制伏隔核PICK1蛋白表达有关。
목적：관찰EGCG대가잡인소서자발활동적영향，병탐토기작용궤제。방법통과소서복강주사가잡인，예선급여EGCG진행간예，채용행위학검측소서적자발활동，채용Western blot 적방법검측소서복격핵PICK1단백적표체。결과EGCG가억제가잡인소치소서자발활동적증강，병차감소소서복격핵 PICK1단백표체。결론EGCG가능대가잡인소치적약물성은유방치작용，기작용가능여억제복격핵PICK1단백표체유관。
Objective The aim of this study was to examine the effect of EGCG on cocaine induced lo-comotor activity in mice ,and to explore its mechanism of action .Methods EGCG was pretreated before cocaine which was used by intraperitoneal injection .Locomotor activity was detected by behavioral test and the expres-sion of PICK1 protein in the nucleus accumbens ( NAc) was tested by Western blot .Results EGCG can inhibit cocaine induced locomotor activity ,and reduce the expression of PICK 1 protein in NAc.Conclusion EGCG may be important pharmocological effect to prevent cocaine induced drug abuse ,which may be related to inhibition of nucleus accumbens PICK 1 protein expression .