中国中西医结合外科杂志
中國中西醫結閤外科雜誌
중국중서의결합외과잡지
CHINESE JOURNAL OF SURGERY OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2013年
6期
639-642
,共4页
陈业刚%杨拓%刘鹏%王建民%孙光
陳業剛%楊拓%劉鵬%王建民%孫光
진업강%양탁%류붕%왕건민%손광
哺乳动物雷帕霉素靶蛋白%核糖体蛋白S6激酶%膀胱尿路上皮癌%蛋白表达
哺乳動物雷帕黴素靶蛋白%覈糖體蛋白S6激酶%膀胱尿路上皮癌%蛋白錶達
포유동물뢰파매소파단백%핵당체단백S6격매%방광뇨로상피암%단백표체
Mammalian target of rapamycin%ribosomal protein S6 kinase%urothelial bladder cancer
目的：探讨哺乳动物雷帕霉素靶蛋白（mTOR）通路蛋白（p-mTOR）、核糖体蛋白S6激酶（p-p70S6K）在膀胱尿路上皮癌中的表达情况及与预后的关系。方法：利用免疫组化染色检测mTOR通路蛋白p-mTOR、p-p70S6K在膀胱尿路上皮癌中的表达情况，分析p-mTOR、p-p70S6K在不同膀胱组织中的表达差异，与膀胱癌临床病理参数的关系及与膀胱尿路上皮癌预后的关系。结果：p-mTOR在膀胱尿路上皮癌中的阳性表达率（43.3%）高于正常黏膜（17.5%），p-mTOR表达阳性率随着肿瘤分级分期的增加相应增加，多发性膀胱尿路上皮癌p-mTOR表达阳性率（70.5%）高于单发组（31.4%），复发组（70.5%）高于未复发组（31.4%，P＜0.01）；p-p70S6K在膀胱尿路上皮癌阳性率（25.9%）高于正常膀胱黏膜（7.5%，P＜0.05），p-p70S6K表达阳性率随着肿瘤分级分期的增加相应增加，多发性膀胱尿路上皮癌p-p70S6K表达阳性率（45.9%）高于单发组（17.1%），复发组（30.3%）高于未复发组（17.4%，P＜0.01）；肿瘤分级、分期、数目及p-mTOR表达分别是膀胱肿瘤无瘤生存的独立预后因子。结论：mTOR/p-mTOR/p-p70S6K通路的激活与膀胱尿路上皮癌的发生发展有关，p-mTOR可以作为预测膀胱尿路上皮癌复发的预后因子。
目的：探討哺乳動物雷帕黴素靶蛋白（mTOR）通路蛋白（p-mTOR）、覈糖體蛋白S6激酶（p-p70S6K）在膀胱尿路上皮癌中的錶達情況及與預後的關繫。方法：利用免疫組化染色檢測mTOR通路蛋白p-mTOR、p-p70S6K在膀胱尿路上皮癌中的錶達情況，分析p-mTOR、p-p70S6K在不同膀胱組織中的錶達差異，與膀胱癌臨床病理參數的關繫及與膀胱尿路上皮癌預後的關繫。結果：p-mTOR在膀胱尿路上皮癌中的暘性錶達率（43.3%）高于正常黏膜（17.5%），p-mTOR錶達暘性率隨著腫瘤分級分期的增加相應增加，多髮性膀胱尿路上皮癌p-mTOR錶達暘性率（70.5%）高于單髮組（31.4%），複髮組（70.5%）高于未複髮組（31.4%，P＜0.01）；p-p70S6K在膀胱尿路上皮癌暘性率（25.9%）高于正常膀胱黏膜（7.5%，P＜0.05），p-p70S6K錶達暘性率隨著腫瘤分級分期的增加相應增加，多髮性膀胱尿路上皮癌p-p70S6K錶達暘性率（45.9%）高于單髮組（17.1%），複髮組（30.3%）高于未複髮組（17.4%，P＜0.01）；腫瘤分級、分期、數目及p-mTOR錶達分彆是膀胱腫瘤無瘤生存的獨立預後因子。結論：mTOR/p-mTOR/p-p70S6K通路的激活與膀胱尿路上皮癌的髮生髮展有關，p-mTOR可以作為預測膀胱尿路上皮癌複髮的預後因子。
목적：탐토포유동물뢰파매소파단백（mTOR）통로단백（p-mTOR）、핵당체단백S6격매（p-p70S6K）재방광뇨로상피암중적표체정황급여예후적관계。방법：이용면역조화염색검측mTOR통로단백p-mTOR、p-p70S6K재방광뇨로상피암중적표체정황，분석p-mTOR、p-p70S6K재불동방광조직중적표체차이，여방광암림상병리삼수적관계급여방광뇨로상피암예후적관계。결과：p-mTOR재방광뇨로상피암중적양성표체솔（43.3%）고우정상점막（17.5%），p-mTOR표체양성솔수착종류분급분기적증가상응증가，다발성방광뇨로상피암p-mTOR표체양성솔（70.5%）고우단발조（31.4%），복발조（70.5%）고우미복발조（31.4%，P＜0.01）；p-p70S6K재방광뇨로상피암양성솔（25.9%）고우정상방광점막（7.5%，P＜0.05），p-p70S6K표체양성솔수착종류분급분기적증가상응증가，다발성방광뇨로상피암p-p70S6K표체양성솔（45.9%）고우단발조（17.1%），복발조（30.3%）고우미복발조（17.4%，P＜0.01）；종류분급、분기、수목급p-mTOR표체분별시방광종류무류생존적독립예후인자。결론：mTOR/p-mTOR/p-p70S6K통로적격활여방광뇨로상피암적발생발전유관，p-mTOR가이작위예측방광뇨로상피암복발적예후인자。
Objective To investigate the expression of p-mTOR and p-p70S6K in human bladder cancer tissues and the correlation with the prognosis. Methods The expression of p-mTOR and p-p70S6K was de-tected in normal bladder tissues and bladder cancer tissues by immunohistochemistry. Their relationship with clinicopathological characteristics and prognosis was analysed by Kaplan-Meier method and Log rank test. Results The expression of p-mTOR in bladder cancer tissues was 43.3%, higher than 17.5% in normal blad-der tussues. The positive rate of p-mTOR expression increased with the progress of pathological stage and tumor grade. The expression of p-mTOR was 70.5% in multiple bladder tumors, higher than 31.4% in single bladder tumor; 70.5% with positive expression of p-mTOR relapsed, higher than 31.4% without recurrence. The expres-sion of p-p70S6K in bladder cancer tissues was 25.9%, higher than 7.5% in normal bladder tussues. The posi-tive rate of p-p70S6K expression increased with the progress of pathological staging and tumor grade. The ex-pression of p-p70S6K was 45.9% in multiple bladder tumors than 17.1% in single bladder tumor; 30.3% with positive expression of p-p70S6K relapsed, higher than 17.4% without recurrence. The difference was statistical significant. The pathological staging, tissue grade, number of tumors and expression of p-mTOR were identified as independent prognostic factors for bladder cancer by univariate and multivariate Cox regression analysis. Conclusion The mTOR/p-mTOR/p-p70S6K pathway was correlated with tumor oncology and tumor progress, p-mTOR protein was an independent prognostic factor for bladder cancer.
