CAJ | 학술논문

目的：研究樱花提取物体外抗氧化的功能及安全性。方法：采用二氯荧光素双乙酸盐（ DCFH-DA）作为荧光探针测定不同浓度樱花提取物（0.5％、1％、2％）处理后的成纤维细胞的荧光强度，同时利用3-（4，5-二甲基噻唑-2）-2，5-二苯基四氮唑溴盐（MTT）法检测不同浓度（0．5％、1％、2％）的樱花提取物对RAW 264．7巨噬细胞存活率的影响，分别以不添加樱花提取物处理的成纤维细胞和巨噬细胞为对照。结果：各不同浓度樱花提取物处理过的成纤维细胞内荧光强度均低于对照组。90分钟时，0.5％、1％、2％的樱花提取物组与对照组相比，荧光强度分别降低了16.55％、19.19％和29.3％（ P值均＜0.05）。同时，MTT实验显示，不同浓度的樱花提取物（0．5％、1％、2％）处理后的RAW 264．7巨噬细胞的存活率分别为100．27％、98．93％和100．53％。结论：樱花提取物具有一定的抗氧化功能，同时对RAW 264．7巨噬细胞无毒性作用，安全性好。
목적：연구앵화제취물체외항양화적공능급안전성。방법：채용이록형광소쌍을산염（ DCFH-DA）작위형광탐침측정불동농도앵화제취물（0.5％、1％、2％）처리후적성섬유세포적형광강도，동시이용3-（4，5-이갑기새서-2）-2，5-이분기사담서추염（MTT）법검측불동농도（0．5％、1％、2％）적앵화제취물대RAW 264．7거서세포존활솔적영향，분별이불첨가앵화제취물처리적성섬유세포화거서세포위대조。결과：각불동농도앵화제취물처리과적성섬유세포내형광강도균저우대조조。90분종시，0.5％、1％、2％적앵화제취물조여대조조상비，형광강도분별강저료16.55％、19.19％화29.3％（ P치균＜0.05）。동시，MTT실험현시，불동농도적앵화제취물（0．5％、1％、2％）처리후적RAW 264．7거서세포적존활솔분별위100．27％、98．93％화100．53％。결론：앵화제취물구유일정적항양화공능，동시대RAW 264．7거서세포무독성작용，안전성호。
Objective:To study the anti-oxidative activity and safety of Cherry Blossom Extracts in vitro.Methods:The fluorescence in fibroblast cells cultured with different concentrations of Cherry Blossom extract (0.5%、1%、2%) was determined using dichlorodihydrofluorescin diace-tate (DCFH-DA) as fluorescence probe, and the RAW264.7 macrophage cell's livability was de-termined by the MTT assay after cultured with different concentrations of Cherry Blossom extract (0.5%、1%、2%).The fibroblast cells and the RAW264.7 macrophage cell without Cherry Blos-som extract was studied as controls respectively .Results:The fluorescence in fibroblast cells cul-tured with different concentrations Cherry Blossom extract was lower than the control group .The fluorescence of 0.5%、1%、2% Cherry Blossom extract group decreased by 16.55%, 19.19%and 29.3%respectively ( all P<0.05 ) at 90 min compared with the control group .Meanwhile , MTT experiments showed that after cultured with different concentrations of Cherry Blossom extract (0.5%, 1%, 2%) , the RAW 264.7 macrophage cell viability was 100.27%, 98.93% and 100.53%respectively .Conclusion:Cherry Blossom extract has anti-oxidation function ,and has no toxic effects to the RAW 264 .7 macrophages cell .