CAJ | 학술논문

利用CTMAB（三甲基十六烷基溴化铵）具有相转移催化和增敏络合体系等特性，建立了用可见分光光度法测定己烯雌酚（DES）的方法。研究表明，DES能将 Fe（Ⅲ）还原成 Fe（Ⅱ），Fe（Ⅱ）与 K3[Fe（CN）6]在CTMAB存在的环境下形成稳定的多元胶束络合体系，其最大吸收波长在736 nm处。DES的质量浓度在0.03256～0.6521 ug/mL范围内与吸光度呈良好线性关系，线性回归方程 A=0.0027+0.3990ρ（μg/mL），相关系数R2=0.9991，摩尔吸光系数ε=5.43×105（L·mol-1·cm-1），检出限1.35μg/mL，相对标准偏差为0.89%，方法用于测定奶粉中DES含量，回收率为89.3%～101.6%。
이용CTMAB（삼갑기십륙완기추화안）구유상전이최화화증민락합체계등특성，건립료용가견분광광도법측정기희자분（DES）적방법。연구표명，DES능장 Fe（Ⅲ）환원성 Fe（Ⅱ），Fe（Ⅱ）여 K3[Fe（CN）6]재CTMAB존재적배경하형성은정적다원효속락합체계，기최대흡수파장재736 nm처。DES적질량농도재0.03256～0.6521 ug/mL범위내여흡광도정량호선성관계，선성회귀방정 A=0.0027+0.3990ρ（μg/mL），상관계수R2=0.9991，마이흡광계수ε=5.43×105（L·mol-1·cm-1），검출한1.35μg/mL，상대표준편차위0.89%，방법용우측정내분중DES함량，회수솔위89.3%～101.6%。
A catalytic and sensitive spectrophotometric method for the determination of diethyl-stilbestrol(DES)with CTMAB has been developed. The method is based on the phase transfer cataly-sis and sensitive complex ation characteristic of CTMAB. The study showed that Fe(Ⅲ)was re-duced to Fe(Ⅱ)by DES and a stable multi-micelle complex was formed by Fe(Ⅱ)and K3[Fe (CN)6]in the existence of CTMAB,the maximum absorption is at at 736 nm. Lambert-Beer law was well obeyed at DES concentrations ranging from 0. 032 56 to 0. 652 1 μg/mL,regression equation was A=0. 002 7+0. 399 0 ρ,correlation coefficient R2 was 0. 999 1,detection limit was 1. 35μg/mL and the molar absorption coefficient wasε=5. 43 × 105(L·mol-1·cm-1);the relative standard devia-tion was 0. 89%. The proposed method was used in the determination of DES in powdered milk and the recovery was 89.3%~101.6%.