中国保健营养(中旬刊)
中國保健營養(中旬刊)
중국보건영양(중순간)
China Hwalth Care & nutrition
2013年
8期
732-733
,共2页
蔡伟江%梁洁仪%Liang Jieyi
蔡偉江%樑潔儀%Liang Jieyi
채위강%량길의%Liang Jieyi
16 种氨基酸%含量测定%液质联用法
16 種氨基痠%含量測定%液質聯用法
16 충안기산%함량측정%액질련용법
目的:建立保健品中16种氨基酸含量的测定方法。方法:采用高效液相色谱串联质谱法,色谱安捷伦 poroshel 120 EC-C18(50mm×4.6mm,2.7μm),以0.35g 乙酸铵加水0.5L 溶解,然后加入0.5L 乙腈,混合,用乙酸调 pH=3.5为流动相,流速0.25ml/min。结果:16种氨基酸的浓度与峰面积线性关系良好,平均回收率在78%~103%之间,RSD≤5%。结论:高效液相色谱串联质谱法快速、灵敏、稳定,可用于保健品中氨基酸的含量测定。
目的:建立保健品中16種氨基痠含量的測定方法。方法:採用高效液相色譜串聯質譜法,色譜安捷倫 poroshel 120 EC-C18(50mm×4.6mm,2.7μm),以0.35g 乙痠銨加水0.5L 溶解,然後加入0.5L 乙腈,混閤,用乙痠調 pH=3.5為流動相,流速0.25ml/min。結果:16種氨基痠的濃度與峰麵積線性關繫良好,平均迴收率在78%~103%之間,RSD≤5%。結論:高效液相色譜串聯質譜法快速、靈敏、穩定,可用于保健品中氨基痠的含量測定。
목적:건립보건품중16충안기산함량적측정방법。방법:채용고효액상색보천련질보법,색보안첩륜 poroshel 120 EC-C18(50mm×4.6mm,2.7μm),이0.35g 을산안가수0.5L 용해,연후가입0.5L 을정,혼합,용을산조 pH=3.5위류동상,류속0.25ml/min。결과:16충안기산적농도여봉면적선성관계량호,평균회수솔재78%~103%지간,RSD≤5%。결론:고효액상색보천련질보법쾌속、령민、은정,가용우보건품중안기산적함량측정。
Objective: To establish the method for content determination of 16 kinds of amino acid in health foods.Methods: Using high performance liquid chromatography tandem mass spectrometry, chromatography Agilent poroshel 120 EC-C18 ( 50mm ×4.6mm, 2.7 μ m ), with 0.35g ammonium acetate with 0.5L solution, then add 0.5L acetonitrile, mixed, with acetic acid to adjust the pH=3.5 as the mobile phase, the flow rate was 0.25ml/min.Results : The linear relationship between concentration and peak area of 16 kinds of amino acids, the average recovery rate was 78%~103%, RSD ≤ 5%.Conclusion: High performance liquid chromatography tandem mass spectrometry method is rapid, sensitive, stable, and can be used for determination of the content of amino acid in health foods.