CAJ | 학술논문

建立了稻谷中有机硒化合物测定方法，采用温和水解富硒大米蛋白的提取条件，利用柱前衍生-反相高效液相色谱测定水解大米蛋白中有机硒含量。结果表明：采用6 mol/L HCl溶液、50℃水解富硒大米蛋白48 h的条件提取率高。以4-氯-3，5-二硝基三氟甲苯（CNBF）为衍生剂的柱前衍生-反相HPLC检测富硒大米中有机硒化合物主要是硒代甲硫氨酸（SeMet），最低检出限为0.14μmol/L，线性范围为0.40~480μmol/L，加标回收率为89.9%~90.8%，硒代甲硫氨酸含量为4.122μg/g，占有机硒含量65.29%。该方法简便、灵敏、重现性好、干扰少，可为稻谷中有机硒测定提供一种完善检测方法。
건립료도곡중유궤서화합물측정방법，채용온화수해부서대미단백적제취조건，이용주전연생-반상고효액상색보측정수해대미단백중유궤서함량。결과표명：채용6 mol/L HCl용액、50℃수해부서대미단백48 h적조건제취솔고。이4-록-3，5-이초기삼불갑분（CNBF）위연생제적주전연생-반상HPLC검측부서대미중유궤서화합물주요시서대갑류안산（SeMet），최저검출한위0.14μmol/L，선성범위위0.40~480μmol/L，가표회수솔위89.9%~90.8%，서대갑류안산함량위4.122μg/g，점유궤서함량65.29%。해방법간편、령민、중현성호、간우소，가위도곡중유궤서측정제공일충완선검측방법。
This paper for the determination of organic selenium in rice by a mid hydrolysis and extraction method to determined by pre-column derivative-RP-HPLC. The results showed that the extraction rate with 100ml of 6mol/L HCl solution,a constant temperature of 50℃,extraction time of 48 h was high. Method of pre-column derivatization-RP-HPLC for the detection of organic selenium compounds which 4-chlorine-3,5-dinitrobenzotrifluoride(CNBF)organic selenium compounds in selenium-enriched rice was mainly selenomethionine(SeMet),The detection limit for selenium was 0.14μmol/L. The linear range was within 0.40~480μmol/L. The recovery was 89.9%~90.8%. contented for 4.122 μg/g selenium in protein,accounting for 65.29% of the selenium content. This method is was the derivatizing agent was simple,rapid and accurate.