CAJ | 학술논문

目的：通过检测胚胎培养液中丙酮酸的含量，评估其预测选择性单胚胎移植妊娠成功率的能力。方法选择到本院不孕中心就诊患者的第3天胚胎培养液，用拉曼光谱技术检测其丙酮酸含量，使用受试工作者曲线下面积评估丙酮酸对胚胎移植妊娠成功率的预测能力。结果妊娠组和非妊娠组第3天培养液中丙酮酸与白蛋白拉曼散射强度比值分别为0.0090（0.0070、0.0189）和0.0238（0.0183、0.0325），差异有统计学意义；丙酮酸结合生态学评分预测妊娠成功的 ROC 曲线下面积为0.846，95％ CI：0.775～0.916，可较好地估计单胚胎移植妊娠率。结论在临床选择优质胚胎时，可考虑使用拉曼光谱法检测胚胎培养液中丙酮酸的含量，以提高选择性单胚胎移植的妊娠成功率。
목적：통과검측배태배양액중병동산적함량，평고기예측선택성단배태이식임신성공솔적능력。방법선택도본원불잉중심취진환자적제3천배태배양액，용랍만광보기술검측기병동산함량，사용수시공작자곡선하면적평고병동산대배태이식임신성공솔적예측능력。결과임신조화비임신조제3천배양액중병동산여백단백랍만산사강도비치분별위0.0090（0.0070、0.0189）화0.0238（0.0183、0.0325），차이유통계학의의；병동산결합생태학평분예측임신성공적 ROC 곡선하면적위0.846，95％ CI：0.775～0.916，가교호지고계단배태이식임신솔。결론재림상선택우질배태시，가고필사용랍만광보법검측배태배양액중병동산적함량，이제고선택성단배태이식적임신성공솔。
Objective To assess the value of pyruvic acid in the embryo culture media in predicting successful pregnancy in elective single embryo transfer (eSET). Methods Patients who came to the infertility center in our hospital were included into the study.Level of pyruvic acid in the embryo culture media was determined.The area under the curve (AUC)of pyruvic acid to predict successful pregnancy was calculated. Results Thirty-four patients were enrolled in the study,and 11 became pregnant,resulting in a rate of 32.35%.No significant differences in age,duration of infertility,causes of infertility,fetal sex,Estradiol,and endometrial thickness were observed between the pregnant group and the non-pregnant group.Pyruvic acid level in the embryo culture media was lower in the pregnant group.The AUC of pyruvic acid to predict successful pregnancy was 0.846 (95% CI:0.775-0.916). Conclusion Pyruvic acid in the embryo culture media may be a predictor to successful pregnancy.