中国保健营养（中旬刊）
中國保健營養（中旬刊）
중국보건영양（중순간）
China Hwalth Care & nutrition
2013年
11期
30-30
,共1页
刘红%候宝贤%郭宝磊%宗丹%关和宇%潘梁%王鹏%裴卓斐
劉紅%候寶賢%郭寶磊%宗丹%關和宇%潘樑%王鵬%裴卓斐
류홍%후보현%곽보뢰%종단%관화우%반량%왕붕%배탁비
目的：探讨氧化应激状态诱导成骨细胞系 MC3T3-E1凋亡中线粒体途径的作用。方法：采用 MTT 法检测不同浓度过氧化氢处理后 MC3T3-E1的增殖情况；Annexin V-FITC/PI流式细胞学检测细胞凋亡；JC-1流式细胞学检测线粒体膜电位；DCFH-DA流式检测ROS活性。结果：MC3T3-E1过氧化氢处理后，细胞增殖明显抑制呈剂量效应关系，ROS 活性和凋亡增加，线粒体膜电位降低。结论：过氧化氢可以增加细胞内 ROS 水平，通过激活线粒体途径诱导细胞凋亡。
目的：探討氧化應激狀態誘導成骨細胞繫 MC3T3-E1凋亡中線粒體途徑的作用。方法：採用 MTT 法檢測不同濃度過氧化氫處理後 MC3T3-E1的增殖情況；Annexin V-FITC/PI流式細胞學檢測細胞凋亡；JC-1流式細胞學檢測線粒體膜電位；DCFH-DA流式檢測ROS活性。結果：MC3T3-E1過氧化氫處理後，細胞增殖明顯抑製呈劑量效應關繫，ROS 活性和凋亡增加，線粒體膜電位降低。結論：過氧化氫可以增加細胞內 ROS 水平，通過激活線粒體途徑誘導細胞凋亡。
목적：탐토양화응격상태유도성골세포계 MC3T3-E1조망중선립체도경적작용。방법：채용 MTT 법검측불동농도과양화경처리후 MC3T3-E1적증식정황；Annexin V-FITC/PI류식세포학검측세포조망；JC-1류식세포학검측선립체막전위；DCFH-DA류식검측ROS활성。결과：MC3T3-E1과양화경처리후，세포증식명현억제정제량효응관계，ROS 활성화조망증가，선립체막전위강저。결론：과양화경가이증가세포내 ROS 수평，통과격활선립체도경유도세포조망。
Objective:To investigate the effect mitochondria signal patway on the apoptosis of MC3T3-E1 induced by hydrogen peroxide. Methods:MC3T3-E1 cells were treated with different doses of hydrogen peroxide. MTT was used to exam the cellinhibition rate. We decected the apoptosis rate, JC-1 the leve of ROS and the expression of Bax in mitochondria. Results:The apoptosis rate and the leve of ROS were increased by hydrogen peroxide, mitochondria membrane potential was deceased. Conclusion:Hydrogen peroxide can induce MC3T3-E1 cellapoptosis through mitochondria signal pathway.
