中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
21期
9485-9488
,共4页
张梦云%周京国%青玉凤%黄翠平%潘舒月%蒲梦君%杨其彬%党万太
張夢雲%週京國%青玉鳳%黃翠平%潘舒月%蒲夢君%楊其彬%黨萬太
장몽운%주경국%청옥봉%황취평%반서월%포몽군%양기빈%당만태
干燥综合征%端粒重复序列结合蛋白质1%端粒重复序列结合蛋白质2
榦燥綜閤徵%耑粒重複序列結閤蛋白質1%耑粒重複序列結閤蛋白質2
간조종합정%단립중복서렬결합단백질1%단립중복서렬결합단백질2
Sjogren's syndrome%Telomeric repeat binding protein 1%Telomeric repeat binding protein 2
目的:端粒重复序列结合蛋白质1(telomeric-repeat binding factor-1,TRF1)和端粒重复序列结合蛋白质2(telomeric-repeat binding factor-2,TRF2)基因表达异常在原发性干燥综合征(primary Sjogren's,pSS)发病中可能的作用。方法采用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)检测55例pSS患者和41例健康对照者(healthy control,HC)外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中TRF1和TRF2转录水平,并与临床指标进行相关性分析。结果(1)PBMCs TRF2转录水平在SS组显著高于HC组(0.0047±0.0107vs.0.0026±0.0049,P=0.001),而TRF1转录水平在两组表达差异无统计学意义(P>0.05)。(2)Spearman相关性分析发现在SS组中TRF2与白细胞计数(WBC)(r=0.309,P<0.05)、中性粒细胞计数(GR)(r=0.312,P<0.05)、补体3(C3)(r=0.470,P<0.05)、抗核抗体(ANA)(r=0.339,P<0.05)呈显著正相关,TRF1转录水平与TRF2(r=1,P<0.01)、WBC(r=0.316,P<0.05)、GR(r=0.313,P<0.05)、ANA(r=0.421, P<0.05)呈显著正相关。结论 TRF2转录水平在SS患者的增高提示其可能参与了SS患者端粒酶激活和端粒缩短的调节;TRF2、TRF1表达水平与 WBC、GR、C3、ANA 呈显著正相关,提示 TRF2、TRF1可能参与了SS炎症免疫调节,且与SS的发病机制有关。加强端粒保护蛋白在SS等自身免疫性疾病的研究有利于进一步阐明这类疾病的发病机制。
目的:耑粒重複序列結閤蛋白質1(telomeric-repeat binding factor-1,TRF1)和耑粒重複序列結閤蛋白質2(telomeric-repeat binding factor-2,TRF2)基因錶達異常在原髮性榦燥綜閤徵(primary Sjogren's,pSS)髮病中可能的作用。方法採用實時熒光定量聚閤酶鏈反應(real-time quantitative polymerase chain reaction,RT-qPCR)檢測55例pSS患者和41例健康對照者(healthy control,HC)外週血單箇覈細胞(peripheral blood mononuclear cells,PBMCs)中TRF1和TRF2轉錄水平,併與臨床指標進行相關性分析。結果(1)PBMCs TRF2轉錄水平在SS組顯著高于HC組(0.0047±0.0107vs.0.0026±0.0049,P=0.001),而TRF1轉錄水平在兩組錶達差異無統計學意義(P>0.05)。(2)Spearman相關性分析髮現在SS組中TRF2與白細胞計數(WBC)(r=0.309,P<0.05)、中性粒細胞計數(GR)(r=0.312,P<0.05)、補體3(C3)(r=0.470,P<0.05)、抗覈抗體(ANA)(r=0.339,P<0.05)呈顯著正相關,TRF1轉錄水平與TRF2(r=1,P<0.01)、WBC(r=0.316,P<0.05)、GR(r=0.313,P<0.05)、ANA(r=0.421, P<0.05)呈顯著正相關。結論 TRF2轉錄水平在SS患者的增高提示其可能參與瞭SS患者耑粒酶激活和耑粒縮短的調節;TRF2、TRF1錶達水平與 WBC、GR、C3、ANA 呈顯著正相關,提示 TRF2、TRF1可能參與瞭SS炎癥免疫調節,且與SS的髮病機製有關。加彊耑粒保護蛋白在SS等自身免疫性疾病的研究有利于進一步闡明這類疾病的髮病機製。
목적:단립중복서렬결합단백질1(telomeric-repeat binding factor-1,TRF1)화단립중복서렬결합단백질2(telomeric-repeat binding factor-2,TRF2)기인표체이상재원발성간조종합정(primary Sjogren's,pSS)발병중가능적작용。방법채용실시형광정량취합매련반응(real-time quantitative polymerase chain reaction,RT-qPCR)검측55례pSS환자화41례건강대조자(healthy control,HC)외주혈단개핵세포(peripheral blood mononuclear cells,PBMCs)중TRF1화TRF2전록수평,병여림상지표진행상관성분석。결과(1)PBMCs TRF2전록수평재SS조현저고우HC조(0.0047±0.0107vs.0.0026±0.0049,P=0.001),이TRF1전록수평재량조표체차이무통계학의의(P>0.05)。(2)Spearman상관성분석발현재SS조중TRF2여백세포계수(WBC)(r=0.309,P<0.05)、중성립세포계수(GR)(r=0.312,P<0.05)、보체3(C3)(r=0.470,P<0.05)、항핵항체(ANA)(r=0.339,P<0.05)정현저정상관,TRF1전록수평여TRF2(r=1,P<0.01)、WBC(r=0.316,P<0.05)、GR(r=0.313,P<0.05)、ANA(r=0.421, P<0.05)정현저정상관。결론 TRF2전록수평재SS환자적증고제시기가능삼여료SS환자단립매격활화단립축단적조절;TRF2、TRF1표체수평여 WBC、GR、C3、ANA 정현저정상관,제시 TRF2、TRF1가능삼여료SS염증면역조절,차여SS적발병궤제유관。가강단립보호단백재SS등자신면역성질병적연구유리우진일보천명저류질병적발병궤제。
ObjectiveTo investigate the expression levels of telomeric protein TRF1 and TRF2 mRNA in the peripheral blood mononuclear cells (PBMCs) of patients with primary Sjogren's syndrome (SS), and the relations between these gene expression levels and disease activity are explored.Methods TRF1 and TRF2 genes were measured using real-time quantitative polymerase chain reaction (RT-qPCR) in PBMCs. The expression levels of TRF1 and TRF2 genes in PBMCs were compared between 55 SS patients and 41 healthy individuals. ResultsThe expression levels of TRF2 in the PBMCs of SS patients significantly increased compared to healthy individuals(0.0047±0.0107vs. 0.0026±0.0049,P=0.001); significant difference was not found in the expression of TRF1. The expression levels of TRF2 was positively correlated with WBC(r=0.309,P<0.05), GR(r=0.312,P<0.05), C3(r=0.470,P<0.05), ANA(r=0.339,P<0.05). The expression levels of TRF1 was positively correlated with TRF2(r=1,P<0.01), WBC(r=0.316,P<0.05), GR(r=0.313,P<0.05), ANA(r=0.421,P<0.05).Conclusion The high expressive of TRF2 mRNA may lead to dysfunction of telomere. TRF1 and TRF2 genes play a key role in the inflammatory immune regulation of SS, and they are involved in the pathogenesis of SS. Further study in expression and functions of telomeric proteins would be needed.