中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
21期
9475-9479
,共5页
王平%张洪洋%彭贤贵%张曦%邓小娟%刘思恒%墙星%冉岑霞%江峰锦
王平%張洪洋%彭賢貴%張晞%鄧小娟%劉思恆%牆星%冉岑霞%江峰錦
왕평%장홍양%팽현귀%장희%산소연%류사항%장성%염잠하%강봉금
白血病%外周血干细胞移植%造血干细胞动员%移植%采集
白血病%外週血榦細胞移植%造血榦細胞動員%移植%採集
백혈병%외주혈간세포이식%조혈간세포동원%이식%채집
Acute leukemia%Peripheral blood stem cell%Hematopoietic stem cell mobilization%Transplantation%Collection
目的：探讨急性白血病患者外周血干细胞动员、采集的效率及影响因素。方法对37例急性白血病患者经化疗＋生长因子动员，动员第5～10天，当外周血WBC＞5×109/L，CD34＋＞20个/μl时，使用CS-3000 Plus血细胞分离机（Baxter公司）进行外周血干细胞采集；采集前外周血WBC分类单个核细胞（MNC，包括幼稚细胞、淋巴细胞及单核细胞），计算MNC%×WBC计数＞（4～6）×109/L预计需要采集循环血容量。分析不同化疗动员时机、疾病、年龄、性别的动员采集，并对采集前外周血进行白细胞计数、分类以及干细胞采集物进行WBC计数、分类和CD34＋检测。结果所有患者均成功动员和采集到了外周血干细胞（MNC＞6×108/kg，CD34＋＞2×106/kg），并成功造血重建。急性髓细胞白血病患者采集所获得的 MNC 与急性淋巴细胞白血病患者采集获得无明显差异；但急性淋巴细胞白血病患者采集获得CD34＋细胞明显较多（P＝0.015）；动员时机的化疗病程与采集物的CD34＋细胞呈负相关，动员时机≤3个疗程与≥6个疗程比较，前者CD34＋细胞明显较好，具有统计学差异（P＝0.028）；外周血MNC数值与采集物MNC及CD34＋细胞具有相关性（r＝0.600，P＝0.00；r＝0.510，P＝0.001）。结论根据不同的疾病、性别、年龄，采用不同的动员采集方案，一般可以成功采集。外周血 MNC 数值对采集物中 CD34＋细胞的总量具有一定预测意义。
目的：探討急性白血病患者外週血榦細胞動員、採集的效率及影響因素。方法對37例急性白血病患者經化療＋生長因子動員，動員第5～10天，噹外週血WBC＞5×109/L，CD34＋＞20箇/μl時，使用CS-3000 Plus血細胞分離機（Baxter公司）進行外週血榦細胞採集；採集前外週血WBC分類單箇覈細胞（MNC，包括幼稚細胞、淋巴細胞及單覈細胞），計算MNC%×WBC計數＞（4～6）×109/L預計需要採集循環血容量。分析不同化療動員時機、疾病、年齡、性彆的動員採集，併對採集前外週血進行白細胞計數、分類以及榦細胞採集物進行WBC計數、分類和CD34＋檢測。結果所有患者均成功動員和採集到瞭外週血榦細胞（MNC＞6×108/kg，CD34＋＞2×106/kg），併成功造血重建。急性髓細胞白血病患者採集所穫得的 MNC 與急性淋巴細胞白血病患者採集穫得無明顯差異；但急性淋巴細胞白血病患者採集穫得CD34＋細胞明顯較多（P＝0.015）；動員時機的化療病程與採集物的CD34＋細胞呈負相關，動員時機≤3箇療程與≥6箇療程比較，前者CD34＋細胞明顯較好，具有統計學差異（P＝0.028）；外週血MNC數值與採集物MNC及CD34＋細胞具有相關性（r＝0.600，P＝0.00；r＝0.510，P＝0.001）。結論根據不同的疾病、性彆、年齡，採用不同的動員採集方案，一般可以成功採集。外週血 MNC 數值對採集物中 CD34＋細胞的總量具有一定預測意義。
목적：탐토급성백혈병환자외주혈간세포동원、채집적효솔급영향인소。방법대37례급성백혈병환자경화료＋생장인자동원，동원제5～10천，당외주혈WBC＞5×109/L，CD34＋＞20개/μl시，사용CS-3000 Plus혈세포분리궤（Baxter공사）진행외주혈간세포채집；채집전외주혈WBC분류단개핵세포（MNC，포괄유치세포、림파세포급단핵세포），계산MNC%×WBC계수＞（4～6）×109/L예계수요채집순배혈용량。분석불동화료동원시궤、질병、년령、성별적동원채집，병대채집전외주혈진행백세포계수、분류이급간세포채집물진행WBC계수、분류화CD34＋검측。결과소유환자균성공동원화채집도료외주혈간세포（MNC＞6×108/kg，CD34＋＞2×106/kg），병성공조혈중건。급성수세포백혈병환자채집소획득적 MNC 여급성림파세포백혈병환자채집획득무명현차이；단급성림파세포백혈병환자채집획득CD34＋세포명현교다（P＝0.015）；동원시궤적화료병정여채집물적CD34＋세포정부상관，동원시궤≤3개료정여≥6개료정비교，전자CD34＋세포명현교호，구유통계학차이（P＝0.028）；외주혈MNC수치여채집물MNC급CD34＋세포구유상관성（r＝0.600，P＝0.00；r＝0.510，P＝0.001）。결론근거불동적질병、성별、년령，채용불동적동원채집방안，일반가이성공채집。외주혈 MNC 수치대채집물중 CD34＋세포적총량구유일정예측의의。
ObjectiveTo investigate the factors influencing the efficiency of mobilization and collection of peripheral hematopoietic stem cells in acute leukemia patients.MethodsAfter mobilization with granulocyte colony stimulating factor (G-CSF) or with combination of G-CSF and chemotheraphy for patients of acute leukemia patients, the peripheral blood hematopoietic stem cells were collected by CS 3000 PLUS blood cell separator. The effect of different diseases, ways of mobilization, age, and sex on the mobilization and collection was analyzed. We did WBC count, morphological classification and CD34+ detection about the peripheral blood stem cell and the peripheral blood before collected the stem cells.ResultsThe collection was successful with all cases, with MNC>6×108/kg, and CD34+>2×106/kg. Compared to AML and ALL, the collected MNC were got no obvious difference. But acute lymphocytic leukemia patients collected more CD34+cells; It had a significant differences (P=0.015). Mobilization of the time point about the number of chemotherapy and acquisition of CD34+ cells was negatively correlated(P=0.028). There were certain relation between the Lymphocyte numerical and the MNC, CD34+cells in PBSC. They had positive correlation.ConclusionIt can successfully collect peripheral hematopoietic stem cells with different protocol according to different diseases, sex and age. To collected in the amount of CD34+cells, peripheral blood lymphocytes of quantity has certain prediction significance.
