CAJ | 학술논문

微卫星序列包含有多种重复单元，在其两碱基重复的类型当中，CT/GA重复类型在功能基因组中比例较高。三疣梭子蟹是重要的养殖品种，对遗传种质的分析需要开发大量的分子标记，本文利用5’锚定引物PCT筛选三疣梭子蟹的CT/AG微卫星分子标记，测序的41个克隆子中，35条序列含有微卫星DNA序列，阳性克隆比率达到85.4%，共检测到55个微卫星位点，分布于20条序列中，其中19条序列检测到2个或2个以上微卫星位点。对所有筛选到的微卫星位点进行分类统计后表明，核心序列为(CT/AG)的有27个(50%)，27个位点为其他类型的重复。结果表明三疣梭子蟹CT/AG微卫星标记在三疣梭子蟹基因组中含量较丰富，5’锚定引物法在筛选特定标记的同时，还筛选了其他类型的微卫星标记，具有较强的筛选效率。
미위성서렬포함유다충중복단원，재기량감기중복적류형당중，CT/GA중복류형재공능기인조중비례교고。삼우사자해시중요적양식품충，대유전충질적분석수요개발대량적분자표기，본문이용5’묘정인물PCT사선삼우사자해적CT/AG미위성분자표기，측서적41개극륭자중，35조서렬함유미위성DNA서렬，양성극륭비솔체도85.4%，공검측도55개미위성위점，분포우20조서렬중，기중19조서렬검측도2개혹2개이상미위성위점。대소유사선도적미위성위점진행분류통계후표명，핵심서렬위(CT/AG)적유27개(50%)，27개위점위기타류형적중복。결과표명삼우사자해CT/AG미위성표기재삼우사자해기인조중함량교봉부，5’묘정인물법재사선특정표기적동시，환사선료기타류형적미위성표기，구유교강적사선효솔。
In this study, CT/AG microsatellite markers were separated using 5’anchored primer PCT in swimming crab genomic DNA. Among the 41 sequenced colons, 35 (85.4%) sequences have microsatellites. A-part from some replications, 55 microsatellites were searched in 20 sequences. And 19 sequences have more than one microsatellite. The classifying for the repeat types of these microsatellites performed that among the 55 microsatellites, 27 microsatellites (50%) were CT/AG type and 27 were other types. The results showed that CT/AG microsatellite was abundant in P. trituberculatus; Additionally some other type microsatellites were iso-lated at the same time using 5’anchored primer. The selection efficiency was raised significantly.