CAJ | 학술논문

目的：探讨未经治疗和随访1、3、5 d手足口患儿外周血单核细胞亚群的变化。方法流式细胞仪检测104例未经治疗手足口患儿（包括阴性患儿62例，EV71阳性患儿37例，CVA16阳性患儿5例）和随访1、3、5 d的患儿外周血单核细胞CD14highCD16-亚群、CD14highCD16+亚群和CD14lowCD16+亚群。结果与健康儿童组相比，手足口患儿CD14highCD16+单核细胞亚群所占总单核细胞比例明显升高（t =4.092，P ＜0.001）；CD14highCD16-亚群比例明显降低。阴性、EV71型、CVA16型患儿之间CD14highCD16-亚群、CD14highCD16+亚群和CD14lowCD16+亚群差异均无统计学意义。结论未经治疗的手足口患儿外周血单核细胞亚群的变化可能与EV71及CVA16病毒的持续感染有关，与病毒种类相关性不明显。
목적：탐토미경치료화수방1、3、5 d수족구환인외주혈단핵세포아군적변화。방법류식세포의검측104례미경치료수족구환인（포괄음성환인62례，EV71양성환인37례，CVA16양성환인5례）화수방1、3、5 d적환인외주혈단핵세포CD14highCD16-아군、CD14highCD16+아군화CD14lowCD16+아군。결과여건강인동조상비，수족구환인CD14highCD16+단핵세포아군소점총단핵세포비례명현승고（t =4.092，P ＜0.001）；CD14highCD16-아군비례명현강저。음성、EV71형、CVA16형환인지간CD14highCD16-아군、CD14highCD16+아군화CD14lowCD16+아군차이균무통계학의의。결론미경치료적수족구환인외주혈단핵세포아군적변화가능여EV71급CVA16병독적지속감염유관，여병독충류상관성불명현。
Objective To analyze the changes in peripheral blood monocyte subpopulation in children with hand-foot-and-mouth disease (HFMD). Methods Flow cytometry was used to detect CD14highCD16-, monocyte subpopulations in peripheral blood from 104 children with untreat HFMD, including 62 cases of latent HFMD, 37 cases of EV71 HFMD and 5 cases of CVA16 HFMD, as well as 25 normal children control. Result Compared with the normal control, the proportion of CD14highCD16+monocytes among total monocytes was significantly elevated (4.0% ± 2.4% vs 9.1% ± 5.4%, t = 4.092, P <0.001), while that of CD14highCD16-monocytes was down-regulated (91.5% ± 5.3% vs 84.7% ± 9.8%,t = 3.070,P< 0.01) in HFMD. No statistical difference was observed in the proportion of CD14highCD16-, CD14highCD16+ and CD14lowCD16+ monocytes among the children with EV71, CVA16 HFMD and those with latent HFMD (t >1.062, all P > 0.05). Conclutions The changes in peripheral blood monocyte subpopulation in children with untreat HFMD may be associated with the permanent infection of enterovirus 71 or Coxsackie virus A16, but have no obvious correlation with the types of virus.