中华实验和临床感染病杂志（电子版）
中華實驗和臨床感染病雜誌（電子版）
중화실험화림상감염병잡지（전자판）
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL INFECTIOUS DISEASES(ELECTRONIC VERSION)
2013年
5期
633-636
,共4页
肖凡%董芳%乔雍%常路丝%张仁雯%成军%魏红山
肖凡%董芳%喬雍%常路絲%張仁雯%成軍%魏紅山
초범%동방%교옹%상로사%장인문%성군%위홍산
GLT25D2%包膜大蛋白%乙型肝炎病毒%糖基化修饰
GLT25D2%包膜大蛋白%乙型肝炎病毒%糖基化脩飾
GLT25D2%포막대단백%을형간염병독%당기화수식
Glt25D2%LHBs%HBV%Glycosyltransferase
目的：在前期研究结果提示Glt25D2与HBV 包膜蛋白大蛋白（LHBs）在体外相互作用基础上证明Glt25D2是否与HBV LHBs分泌相关。方法采用激光共聚焦方法分析Glt25D2与LHBs 在HepG2细胞内的定位。免疫共沉淀方法进一步证实Glt25D2与LHBs 的相互作用。采用实时荧光定量PCR和Western blot方法分析mRNA和蛋白表达水平。应用ELISA方法检测细胞上清LHBs水平。实时荧光定量PCR方法检测上清HBV 病毒载量。ELISA方法检测上清HBV LHBs水平。Western blot方法检测细胞中LHBs蛋白含量。Cobas Amplicor HBV Monitor Test方法检测细胞上清液HBV DNA载量。结果Glt25D2与LHBs在体外相互作用，上调的Glt25D2高表达促进HBV DNA复制和LHBs表达，Glt25D2低表达抑制HBV DNA复制和LHBs表达。结论 Glt25D2与乙型肝炎包膜蛋白大蛋白的分泌有关。
目的：在前期研究結果提示Glt25D2與HBV 包膜蛋白大蛋白（LHBs）在體外相互作用基礎上證明Glt25D2是否與HBV LHBs分泌相關。方法採用激光共聚焦方法分析Glt25D2與LHBs 在HepG2細胞內的定位。免疫共沉澱方法進一步證實Glt25D2與LHBs 的相互作用。採用實時熒光定量PCR和Western blot方法分析mRNA和蛋白錶達水平。應用ELISA方法檢測細胞上清LHBs水平。實時熒光定量PCR方法檢測上清HBV 病毒載量。ELISA方法檢測上清HBV LHBs水平。Western blot方法檢測細胞中LHBs蛋白含量。Cobas Amplicor HBV Monitor Test方法檢測細胞上清液HBV DNA載量。結果Glt25D2與LHBs在體外相互作用，上調的Glt25D2高錶達促進HBV DNA複製和LHBs錶達，Glt25D2低錶達抑製HBV DNA複製和LHBs錶達。結論 Glt25D2與乙型肝炎包膜蛋白大蛋白的分泌有關。
목적：재전기연구결과제시Glt25D2여HBV 포막단백대단백（LHBs）재체외상호작용기출상증명Glt25D2시부여HBV LHBs분비상관。방법채용격광공취초방법분석Glt25D2여LHBs 재HepG2세포내적정위。면역공침정방법진일보증실Glt25D2여LHBs 적상호작용。채용실시형광정량PCR화Western blot방법분석mRNA화단백표체수평。응용ELISA방법검측세포상청LHBs수평。실시형광정량PCR방법검측상청HBV 병독재량。ELISA방법검측상청HBV LHBs수평。Western blot방법검측세포중LHBs단백함량。Cobas Amplicor HBV Monitor Test방법검측세포상청액HBV DNA재량。결과Glt25D2여LHBs재체외상호작용，상조적Glt25D2고표체촉진HBV DNA복제화LHBs표체，Glt25D2저표체억제HBV DNA복제화LHBs표체。결론 Glt25D2여을형간염포막단백대단백적분비유관。
Objective Our previous study demonstrated that Glt25D2 interacts with HBV LHBs in vitro. In this study, we aim to prove if Glt25D2, an O-glycosyltransferase, is related with the secretion of hepatitis B virus surface large protein (LHBs). Methods Confocal microscopy was used to determine the co-location between Glt25D2 and LHBs in HepG2 cells. The interaction between Glt25D2 and LHBs in vitro was examined by the method of co-immunoprecipitation. Quantitative real-time PCR and Western blot were undertaken to evaluate the levels of mRNA and protein. The levels of LHBs in the supernatant were measured with ELISA kits and HBV DNA was quantified with the Cobas Amplicor HBV Monitor Test. Results Our data demonstrated that Glt25D2 interactes with LHBs in vitro, up-regulated Glt25D2 expression could increase HBV DNA and LHBs levels in HepG2.2.15, down-regulated Glt25D2 expression decreased HBV DNA and LHBs levels. Conclusions Our results suggest that Glt25D2 is related with HBV LHBs secretion.
