CAJ | 학술논문

目的：探讨人类免疫缺陷病毒（HIV）感染者血清IL-25水平与机会性感染的关系。方法收集55例HIV感染者的临床资料，并应用ELISA法检测血清IL-25浓度，流式细胞术检测CD4+ T细胞计数，分析两者与HIV感染者发生机会性感染的相关性。结果55例患者中有44例（80.00%）发生机会性致病菌感染，其中26例（47.27%）发生口腔念珠菌感染。发生机会性感染组患者血清IL-25水平较未发生感染组高[（946.45±1652.84）pg/ml vs （107.58±167.33）pg/ml，P ＜0.01），CD4+ T细胞计数降低[（87.54±110.60）cell/μl vs （298.60±211.91）cell/μl，P ＜0.05）。发生口腔念珠菌感染组较未发生感染组患者血清IL-25增高[（1247.78±1877.48）pg/ml vs （358.09±944.31）pg/ml，P＜0.05），CD4+ T细胞计数降低[（48.56±74.90）cell/μl vs （200.04±182.79）cell/μl，P ＜0.05）。相关性分析显示，IL-25浓度、CD4+ T细胞计数分别与发生机会性感染、口腔白色念珠菌感染呈正相关、负相关（P ＜0.01）。结论 IL-25可能参与HIV感染者发生机会性感染后的免疫反应，高IL-25水平可能与HIV感染者易患机会性感染、口腔念珠菌感染有关。
목적：탐토인류면역결함병독（HIV）감염자혈청IL-25수평여궤회성감염적관계。방법수집55례HIV감염자적림상자료，병응용ELISA법검측혈청IL-25농도，류식세포술검측CD4+ T세포계수，분석량자여HIV감염자발생궤회성감염적상관성。결과55례환자중유44례（80.00%）발생궤회성치병균감염，기중26례（47.27%）발생구강념주균감염。발생궤회성감염조환자혈청IL-25수평교미발생감염조고[（946.45±1652.84）pg/ml vs （107.58±167.33）pg/ml，P ＜0.01），CD4+ T세포계수강저[（87.54±110.60）cell/μl vs （298.60±211.91）cell/μl，P ＜0.05）。발생구강념주균감염조교미발생감염조환자혈청IL-25증고[（1247.78±1877.48）pg/ml vs （358.09±944.31）pg/ml，P＜0.05），CD4+ T세포계수강저[（48.56±74.90）cell/μl vs （200.04±182.79）cell/μl，P ＜0.05）。상관성분석현시，IL-25농도、CD4+ T세포계수분별여발생궤회성감염、구강백색념주균감염정정상관、부상관（P ＜0.01）。결론 IL-25가능삼여HIV감염자발생궤회성감염후적면역반응，고IL-25수평가능여HIV감염자역환궤회성감염、구강념주균감염유관。
Objective To investigate the relationships of serum concentration of interleukin-25 (IL-25) and opportunistic infections of patients with HIV infection. Methods The serum samples and the clinical information of 55 cases with HIV infection were collected. The serum concentrations of IL-25 and CD4+ T-cell count were measured by enzyme linked immunosorbent assay (ELISA) and flow cytometry, respectively. Results There were 44 cases (80.00%) suffering with opportunistic infections among 55 cases and 26 cases (47.27%) were infected with oral candidiasis. Higher serum level of IL-25 [(946.45 ± 1652.84) pg/ml vs (107.58 ± 167.33) pg/ml, P < 0.01] and lower CD4+ T-cell count [(48.56 ± 74.90 cell/μl vs (200.04 ± 182.79) cell/μl, P < 0.01] were found in patients with opportunistic infections than the cases without opportunistic infections. The serum IL-25 was higher in cases with oral candidiasis than the others [(1247.78 ± 1877.48) pg/ml vs (358.09 ± 944.31) pg/ml, P < 0.05], while the CD4+ T-cell count was significantly lower in patients with oral candidiasis than that in patients without candidiasis [(48.56 ± 74.90) cell/μl vs (200.04 ± 182.79) cell/μl, P < 0.01]. Serum IL-25 and CD4+ T-cell count were positive and negative correlated with opportunistic infection and oral candidiasis, respectively. Conclusions IL-25 may play a role in the immunologic mechanism of HIV patients with opportunistic infections. Higher serum IL-25 is probably related to high susceptibility of opportunistic infection and oral candidiasis.