中华实验和临床感染病杂志(电子版)
中華實驗和臨床感染病雜誌(電子版)
중화실험화림상감염병잡지(전자판)
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL INFECTIOUS DISEASES(ELECTRONIC VERSION)
2013年
6期
825-829
,共5页
梁金秋%庞琳%李洪杰%孟一星%曹金凤%何明%王琦%张锦前%刘顺爱%成军
樑金鞦%龐琳%李洪傑%孟一星%曹金鳳%何明%王琦%張錦前%劉順愛%成軍
량금추%방림%리홍걸%맹일성%조금봉%하명%왕기%장금전%류순애%성군
手足口病%逆转录聚合酶链反应%肠道病毒
手足口病%逆轉錄聚閤酶鏈反應%腸道病毒
수족구병%역전록취합매련반응%장도병독
Hand,mouth and foot disease (HFMD)%Reverse transcription-polymerase chain reaction (RT-PCR)%Enterovirus
目的:了解2011年北京大学北京地坛医院教学医院儿童手足口病住院患者的病原体分布情况及变化趋势,为手足口病的预防、诊断及治疗等提供依据。方法本研究共收集288例手足口病患者的咽拭子标本,使用肠道病毒通用型、肠道病毒71型和柯萨奇16型核酸检测试剂盒应用实时荧光逆转录-聚合酶链反应法(real-time PCR)进行检测。结果2011年度本院肠道病毒阳性标本占51.74%(149/288)。病毒分型结果显示,EV71阳性标本占总标本例数的23.26%(67/288);CoxA16阳性标本占7.29%(21/288);非EV71非CoxA16型阳性标本占21.18%(61/288)。2009至2011年北京地区的手足口病病原学分析结果显示,3年间EV71阳性率变化并不明显;CoxA16阳性率显著下降;非EV71非CoxA16型阳性率显著升高。结论2011年度本院手足口病患儿在主要流行期以EV71和非EV71非CoxA16型肠道病毒感染为主;北京地区2011年手足口病病原学和前两年相比非EV71非CoxA16型感染比例继续升高。
目的:瞭解2011年北京大學北京地罈醫院教學醫院兒童手足口病住院患者的病原體分佈情況及變化趨勢,為手足口病的預防、診斷及治療等提供依據。方法本研究共收集288例手足口病患者的嚥拭子標本,使用腸道病毒通用型、腸道病毒71型和柯薩奇16型覈痠檢測試劑盒應用實時熒光逆轉錄-聚閤酶鏈反應法(real-time PCR)進行檢測。結果2011年度本院腸道病毒暘性標本佔51.74%(149/288)。病毒分型結果顯示,EV71暘性標本佔總標本例數的23.26%(67/288);CoxA16暘性標本佔7.29%(21/288);非EV71非CoxA16型暘性標本佔21.18%(61/288)。2009至2011年北京地區的手足口病病原學分析結果顯示,3年間EV71暘性率變化併不明顯;CoxA16暘性率顯著下降;非EV71非CoxA16型暘性率顯著升高。結論2011年度本院手足口病患兒在主要流行期以EV71和非EV71非CoxA16型腸道病毒感染為主;北京地區2011年手足口病病原學和前兩年相比非EV71非CoxA16型感染比例繼續升高。
목적:료해2011년북경대학북경지단의원교학의원인동수족구병주원환자적병원체분포정황급변화추세,위수족구병적예방、진단급치료등제공의거。방법본연구공수집288례수족구병환자적인식자표본,사용장도병독통용형、장도병독71형화가살기16형핵산검측시제합응용실시형광역전록-취합매련반응법(real-time PCR)진행검측。결과2011년도본원장도병독양성표본점51.74%(149/288)。병독분형결과현시,EV71양성표본점총표본례수적23.26%(67/288);CoxA16양성표본점7.29%(21/288);비EV71비CoxA16형양성표본점21.18%(61/288)。2009지2011년북경지구적수족구병병원학분석결과현시,3년간EV71양성솔변화병불명현;CoxA16양성솔현저하강;비EV71비CoxA16형양성솔현저승고。결론2011년도본원수족구병환인재주요류행기이EV71화비EV71비CoxA16형장도병독감염위주;북경지구2011년수족구병병원학화전량년상비비EV71비CoxA16형감염비례계속승고。
Objective To investigate the pathogen distribution of patients with hand, foot and mouth diseases (HFMD) hospitalized in Division of Pediatrics, Beijing Ditan Hospital in 2011. Methods Real-time fluorescence quantitative RT-PCR kits with three reagents: universal enterovirus primer, coxsackievirus A16 (CoxA16) primers and enterovirus 71 (EV71) primers were used to detect the samples, respectively. Results The enterovirus-positive rate was 51.74% (149/288). Among this, the positive rate of EV71, CoxA16 and other types of entervirus were 23.26% (67/288), 7.29% (21/288), 21.18% (61/288), respectively. And the study reveals that the positive rate of CoxA16 had significantly decreased, EV71 changed little, other types of entervirus significantly increased, during 2009-2011. Conclusions In extensively epidemic-period of 2011, the etiology of children with hand, foot and mouth disease hospitalized in our hospital was dominated alternately by EV71 and non-EV71, non-CoxA16 virus. The positive rate of non-EV71, non-CA16 virus in Beijing area has been persistently increased during 2009-2011.